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HSP70 | Heat shock protein 70 (cytoplasmic)

AS08 371 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. juncea, B. oleracea, C. sativus, C. reinhardtii, D. subspicatus, E. tef, G. vermiculophylla, H. vulgare, M. sativa, O. sativa, P. kurroa, P. strobus, Salicornia sp., S. italica. S. vulgaris, S. lycopersicum, Trebouxia TR1 and TR9, T. aestivum, Z. mays, P. falciparum, V. faba

Benefits of using this antibody

HSP70 | Heat shock protein 70 (cytoplasmic) in the group Antibodies Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS08 371)
HSP70 | Heat shock protein 70 (cytoplasmic)



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Product Information

Immunogen
KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 UniProt: F4KCE5 , HSC70-2 UniProt: A0A178UTH3 and HSC70-3 Uniprot: O65719 as well as heat shock inducible Hsp70 of Arabidopsis thaliana TAIR: AT3g12580/T2E22_110 and At1g16030 and AT3g12580/T2E22_110
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1 : 3000 - 1: 10 000, 5 µg protein/well (WB), 2-3 µl/protein extract of concentration 3-5 mg/ml
Expected | apparent MW

70 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica juncea, Brassica oleracea, Chlamydomonas reinhardtii, Chlamydomonas sp. UWO241, Cucumis sativus, E. teft, Fagopyrum esculentum, Hordeum vulgare, Medicago sativa, Oryza sativa, Salicornia sp., Silene vulgaris, Solanum lycopersicum, Picrorhiza kurroa, Pinus strobus, Polyscias elegans, Zea mays, algae: Desmodesmus subspicatus, Gracilaria vermiculophylla, phycobiont: Trebouxia TR1 and TR9, Plasmodium falciparum, Setaria italica, Triticum aestivum, Ulva prolifera, Vicia faba
Predicted reactivity Ageratina adenophora, Allium sativum, Arabis alpina, Arachis diogoi, Arundo donax, Brassica napus, brassica rapa subsp. pekinensis, Camellia sinensis, Citrus sinensis, Coffea arabica, Eriobotrya japonica, Gossypium arboretum, Glycine max, Glycine soja, Helianthus annuus, Hordeum vulgare var. distichum, Lotus japonicus, Medicago sativa, medicago truncatula, Musa acuminata subsp. malaccensis, Nannochloropsis gaditana, Nicotiana tabacum, Nicotiana bethamiana, Phaseolus vulgaris, Physcomitrium patens, Pinus taeda, Pisum sativum, Populus balsamifera, Populus trichocarpa, Salix gilgiana, Saussurea medusa,Solanum tuberosum, Solanum commersonii, Spinacia oleracea, Tragopogon dubius, Tragopogon porrifolius, Triticum aestivum, Vitis vinifera, Volvox sp.
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application example

western blot image


1µg of total protein
from Horderum vulgare pre heat shock leaf (1), Horderum vulgare post heat shock (2h 40ºC) (2), Zea mays pre heat shock total protein leaf (3), Zea mays post heat shock (2h 40ºC) (4), total protein leaf extracted with Agrisera Protein Eextraction Buffer (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF (Milipore). Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-HSP70 antibody (AS08 371, 1:20 000, 1h) and secondary anti-rabbit (1:20 000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent with extreme femtogram range.



western blot detection using anti-hsp70 antibody

Protein from Solanum lycopersicum (1) total cell extract ca. 30 -50 µg, (2) and (3) nuclei pellet , (4) and (5) ca. 7 µg of nuclei fraction, (6) and (7) cytoplasmic pellet, (8) ca. 7 µg of cytoplasm fraction, were separated on 10% SDS-PAGE and blotted 1h to nitrocellulose (Schleicher & Schuell). Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-HSP70 antibody (AS08 371, 1:5000, 3h RT). The antibody solution was decanted and the blot was rinsed briefly. Washed 3 times for 15 min in TBS-T at room temperature with agitation. Blot was incubated with a secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 5:000. The blot was washed as above and developed for 1 min with ECL detection reagent according to the manufacturers instructions.

Courtesy Dr Rena Gorovits, The Hebrew University of Jerusalem, Israel
 


 western blot using plant anti-HSP70 antibodies

200 fmoles of HSP70 protein standard product number AS08 371S (1), 1 µg of total protein from samples such as Lycopersicum esculentum leaf (2), Nicotiana tabaccum leaf, (3)Zea mays leaf (4), Hordeum vulgare leaf (5), Arabidopsis thaliana leaf (6) were extracted with Agrisera Protein Extraction Buffer PEB (AS08 300). Samples were diluted with 1X sample buffer (NuPAGE LDS sample buffer (Invitrogen) supplemented with 50 mM DTT and heat at 70°C for 5 min and keept on ice before loading. Protein samples were separated on 4- 12% Bolt Plus gels,  LDS-PAGE and blotted for 70 minutes to PVDF using tank transfer. Blots were blocked immediately following transfer in 2% blocking reagent or 5% non-fat milk dissolved in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 (in blocking reagent) for 1h/RT  with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed 1x15 min and 3x5 min with TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS10 1489, Agrisera) diluted to 1:25 000 in blocking reagent for 1h at room temperature with agitation. The blots were washed as above. The blot was developed for 5 min with chemiluminescence detection reagent in extreme femtogram range,  according the manufacturers instructions. Images of the blots were obtained using a CCD imager (VersaDoc MP 4000) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.

Reactant: Plasmodium falciparum

Application: Western Blotting

Pudmed ID: 25058848

Journal: Nat Commun

Figure Number: 5A

Published Date: 2014-07-24

First Author: Guggisberg, A. M., Park, J., et al.

Impact Factor: 13.783

Open Publication

Loss of PfHAD1 is required for FSM resistanceData shown are representative of at least three independent experiments. (a) Immunoblot of the parent strain, FSMR strain AM1, and AM1 Hsp110:PfHAD1-GFP. Full blots are shown in Supplementary Fig. 3. Marker units are kilodaltons (kDa). Top panel was probed with anti-PfHAD1 antisera, and the bottom panel was probed with anti-heat shock protein 70 (hsp70) antisera as a loading control. Expected approximate protein masses: native PfHAD1, 33 kDa; PfHAD1-GFP, 60 kDa; hsp70, 74 kDa. (b) FSM IC50s of the parent strain, FSMR strain AM1, and AM1 Hsp110:PfHAD1-GFP. Displayed are the means ą standard error of the mean (SEM). The parent strain has a FSM IC50 of 1.1 ą 0.2 ?M, while AM1 has an IC50 of 5.5 ą 1.5 ?M. Expression of PfHAD1-GFP in AM1 results in an IC50 of 1.4 ą 0.2 ?M. Data shown are mean is normalized such that 100% growth is defined by that of untreated cells, and 0% growth is defined as the smallest value in each data set.


Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 30042778

Journal: Front Plant Sci

Figure Number: 4A

Published Date: 2018-07-26

First Author: Law, Y. S., Ngan, L., et al.

Impact Factor: 5.435

Open Publication

Co-immunoprecipitation assays of pF1?-1. The binding of RRL-pF1?-1 and RRL-1-77aaF1?-1 + mMORF3 to Hsp70 and 14-3-3 were strongly enhanced by LE treatment.


Reactant: Plasmodium falciparum

Application: Western Blotting

Pudmed ID: 30425143

Journal: MBio

Figure Number: 5A

Published Date: 2018-11-13

First Author: Guggisberg, A. M., Frasse, P. M., et al.

Impact Factor: 6.689

Open Publication

Loss of HAD2 is necessary for FSM resistance. (A) Successful expression of pTEOE110:HAD2-GFP in strain R2 (had2R157X, PFK9) was confirmed by immunoblotting. Marker units are indicated in kilodaltons (kDa). The top blot was probed with anti-HAD2 antiserum (expected masses: HAD, 33?kDa; HAD2-GFP, 60?kDa). The bottom blot was probed with anti-heat shock protein 70 (Hsp70) antiserum as a loading control. (B) Representative FSM dose response demonstrating that expression of HAD2-GFP in strain R2 (had2R157X PFK9) resulted in restored sensitivity to FSM. Strain R2 had an elevated FSM IC50 compared to the parental (par) strain. When HAD2 expression was restored in strain R2, the resulting strain showed an IC50 near that of the parent strain. Data shown are from a representative clone (clone 1) of the HAD2-rescued strain. Additional clones displayed a similar phenotype (see Fig. S2).


Reactant: Plasmodium falciparum

Application: Western Blotting

Pudmed ID: 34182772

Journal: MBio

Figure Number: 7A

Published Date: 2021-06-29

First Author: Mathews, E. S., Jezewski, A. J., et al.

Impact Factor: 6.689

Open Publication

Localization of GAPDH, but not its glycolytic function, is IPP- or farnesylation-dependent. (A to C) Inhibition of IPP synthesis and farnesylation reduced membrane association of GAPDH. (A) Representative anti-GAPDH immunoblots of control-, FSM (20??M)-, and FTI (10??M)-treated P. falciparum. (B and C) Quantification of several immunoblots adjusted with loading control. Anti-Hsp70 (1:5,000) and anti-PM-V (1:500) were used as loading controls for total lysate and membrane fractions, respectively. n?=?5 to 6; ****, P???0.0001, unpaired t test with Welch’s correction.

Additional information

Additional information This product can be sold containing ProClin if requested

Can be sold containing 0.1% ProClin if requested

This antibody can be used as a marker of cytoplasmic fraction in tomato (Anfoka et al. 2015).

Applied primary antibody dilution in western blot depends upon sensitivity of detection reagents (pico or femtogram for chemiluminescent detection).

Immunoprecipitation protocol using Agrisera anti-Hsp70 cytosolic antibodies, see tab: protocols.

Related products

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AS08 348 | Anti-chloroplastic HSP70, rabbit antibodies
AS08 347 | Anti-mitochondrial HSP70, rabbit antibodies

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.

Product citations

Selected references Cvetkovska et al. (2022) A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonas sp. UWO241. Plant, Cell & Environment, 45, 156– 177. https://doi.org/10.1111/pce.14203
Wang et al. (2022) 17-(Allylamino)-17-demethoxygeldanamycin treatment induces the accumulation of heat shock proteins and alleviates senescence in broccoli. Postharvest Biology and Technology,Volume 186, 2022, 111818, ISSN 0925-5214, https://doi.org/10.1016/j.postharvbio.2021.111818.
Swetha et al. (2021) Single and Combined Salinity and Heat Stresses Impact Yield and Dead Pericarp Priming Activity. Plants (Basel). 2021 Aug 8;10(8):1627. doi: 10.3390/plants10081627. PMID: 34451672; PMCID: PMC8399105.
Shteinberg et al. (2021) Tomato Yellow Leaf Curl Virus (TYLCV) Promotes Plant Tolerance to Drought. Cells. 2021 Oct 25;10(11):2875. doi: 10.3390/cells10112875. PMID: 34831098; PMCID: PMC8616339.
Kumari et al. (2021) In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry. BMC Plant Biol. 2021 Dec 22;21(1):604. doi: 10.1186/s12870-021-03394-8. PMID: 34937558; PMCID: PMC8693493.
Mishra et al. (2021) Interplay between abiotic (drought) and biotic (virus) stresses in tomato plants. Mol Plant Pathol. 2021 Dec 30. doi: 10.1111/mpp.13172. Epub ahead of print. PMID: 34970822.
Tabassum et al. (2020). FLOURY ENDOSPERM11-2 Encodes Plastid HSP70-2 Involved With Temperature-Dependent Chalkiness of Rice (Oryza Sativa L.) Grains. Plant J. 10.1111/tpj.14752
Sadura et al. (2020). HSP Transcript and Protein Accumulation in Brassinosteroid Barley Mutants Acclimated to Low and High Temperatures . Int J Mol Sci . 2020 Mar 10;21(5):1889.doi: 10.3390/ijms21051889.
Azaiez et al. (2020). Salt Stress Induces Differentiated Nitrogen Uptake and Antioxidant Responses in Two Contrasting Barley Landraces from MENA Region. Agronomy 10, no. 9: 1426.
P?a?ek et al. (2020). Synthesis of heat-shock proteins HSP-70 and HSP-90 in flowers of common buckwheat (Fagopyrum esculentum) under thermal stress. Crop and Pasture Science, 71(8), 760-767, July 2020
McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263
Deng et al. (2019). Integrated proteome analyses of wheat glume and awn reveal central drought response proteins under water deficit conditions. J Plant Physiol. 2019 Jan;232:270-283. doi: 10.1016/j.jplph.2018.11.011.
Rowarth et al. (2019). Hsp70 plays a role in programmed cell death during the remodelling of leaves of the lace plant (Aponogeton madagascariensis). J Exp Bot. 2019 Nov 6. pii: erz447. doi: 10.1093/jxb/erz447
Lentini et al. (2018). Early responses to cadmium exposure in barley plants: effects on biometric and physiological parameters. Acta Physiologiae Plantarum October 2018, 40:178
Fan et al. (2018). Comparative proteomic analysis of Ulva prolifera response to high temperature stress. Proteome Sci. 2018 Oct 27;16:17. doi: 10.1186/s12953-018-0145-5.
Pan et al. (2018). Comparative proteomic investigation of drought responses in foxtail millet. BMC Plant Biol. 2018 Nov 29;18(1):315. doi: 10.1186/s12870-018-1533-9.
Lentini et al. (2018). Early responses to cadmium exposure in barley plants: effects on biometric and physiological parameters. Acta Physiol Plant (2018) 40: 178. https://doi.org/10.1007/s11738-018-2752-2.
Balážová et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013.
Yoon et al. (2018). The subfamily II catalytic subunits of protein phosphatase 2A (PP2A) are involved in cortical microtubule organization. Planta. 2018 Sep 6. doi: 10.1007/s00425-018-3000-0.
Alamri et al. (2018). Nitric oxide-mediated cross-talk of proline and heat shock proteins induce thermotolerance in Vicia faba L. Environmental and Experimental Botany Available online 23 June 2018.
Barghetti et al. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes Dev. 2017 Nov 15;31(22):2282-2295. doi: 10.1101/gad.301242.117.
Gorovits et al. (2017). The six Tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation. Cell Stress Chaperones. 2017 Mar 21. doi: 10.1007/s12192-017-0766-0.
Fernández-Bautista N. et al. (2017). AtHOP3, a member of the HOP family in Arabidopsis, interacts with BiP and plays a major role in the ER stress response. Plant Cell Environ. 2017 Feb 2. doi: 10.1111/pce.12927.
Hammann et al. (2016). Selection of heat?shock resistance traits during the invasion of the seaweed Gracilaria vermiculophylla. Marine Biology 163: 104.
McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.
Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print]
Gorovits et al. (2016). Tomato yellow leaf curl virus confronts host degradation by sheltering in small/midsized protein aggregates. Virus Res. 2016 Feb 2;213:304-13. doi: 10.1016/j.virusres.2015.11.020. Epub 2015 Dec 1.
Ghandi et al. (2016). Tomato yellow leaf curl virus infection mitigates the heat stress response of plants grown at high temperature. Sci Rep. 2016 Jan 21;6:19715. doi: 10.1038/srep19715.

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