HSP70 | Heat shock protein 70 (chloroplastic)
AS08 348 | Clonality:Polyclonal | Host:Rabbit | Reactivity: A. thaliana, O. sativa, P. sativm, P. strobus

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application information |
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Recommended dilution | 1 : 100 (IP), 1 : 2000 (WB) | |
Expected | apparent MW | 76 | 70 kDa |
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Confirmed reactivity | Arabidopsis thaliana, Hordeum spontaneum, Oryza sativa, Pinus strobus, Pisum sativum |
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Predicted reactivity | Arundo donax, Brachypodium distachyon, Brassica rapa subsp. pekinensis, Brassica napus, Capsella rubella, Citrus clementina, Citrus sinensis, Coffea canephora, Glycine max, Glycine soja, Hordeum vulgare, Medicago trancatula, Oryza sativa, Phaseolus vulgaris, Physomitrella patensm, Picea sitchemsis, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum tuberosum, Solanum lycopersicum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum aestivum, Zea mays, Vitis vinifera | |
Not reactive in | No confirmed exceptions from predicted reactivity are currently known. | |
Additional information | ||
Selected references | Wu et al. (2018). Control of Retrograde Signaling by Rapid Turnover of GENOMES UNCOUPLED 1. Plant Physiol. 2018 Jan 24. pii: pp.00009.2018. doi: 10.1104/pp.18.00009. Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print] Jedmowski et al. (2014). Comparative analysis of drought stress effects on photosynthesis of Eurasian and North African genotypes of wild barley. Photosynthetica, September 2014. |
application example
Total protein from Arabidopsis thaliana chloroplasts (20 µg) and ,Arabidopsis thaliana leaf extracts (25 µg) were separated on 10% acrilamide gel and electrophoresis prepared according to Schägger and von Jagov (Anl. Biochem., 1987, 166:368-379). After running the gel, proteins were transferred to nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Transfer was checked by Ponceau S staining. Blot was destained by several quick washings in distilled water and 1 washing in 1X TBS (10 mM T pH 7.5, 150 mM NaCl) (10-15 min.).Blot was blocked by 1.5 hour in 5% milk in TBST (1X TBS, 0,1 20) After blocking blot was washed quickly twice in TBST and incubated 2 hours with primary antibody (dilution 1: 2000 TBST (dilution 1:1000). Washing: two quick washings in TBST and 3 x 10 min. washings in TBST. Then blot was incubated 45-60 min. with a secondary anti-rabbit antibodies conjugated to peroxidase (dilution 1:10000) in TBST. Washing: as above. After washing blot was incubated 1-2 min. in ECL solution and exposed to Kodakautoradiography film. Exposure time was 10 seconds. Courtesy Dr. J. Piechota
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