Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit antibodies)

Auroy Pascaline
I use this antibody on Chlamydomonas, concentration 1/2500, on N deprivation condition , I see signal with 2.5µg of total protein.	2022-02-24 @ 09:59:46
Manuela Leonardelli
Loaded about 3 ug of protein from Arabidopsis thaliana seedlings. Signal is very clear and clean. No unspecific bands. Exposure time <1min. Used as loading control	2019-06-18 @ 14:33:29
Michele Grieco
For all these antibodies I loaded an amount of sample with a chlorophyll concentration of only 0,5 µl/ml, and in western blotting analyses the ECL signal was very intense. Moreover, I did not get aspecific bands for any of these antibodies.	2014-06-16 @ 09:30:35
Andre Nordhues/Marita Hermann
50 µg of total protein from Chlamydomonas reinhardtii was separated on 13 % SDS Gel and blotted 1h to PVDF.Blots were incubated in the primary antibody at a dilution of 1: 6 000 for 1h at room temperature with agitation.The primary antibody can be reused several times. Three bands are detected: 70 kDa (chloroplastic CF1 Atp Synthase), ~90 kDa, and ~120 kDa.	2012-11-27 @ 17:27:16