Anti-PsaK | PSI-K subunit of photosystem I

Product no: AS23 4919

AS23 4919 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PsaK protein, UniProt: Q9SUI5 TAIR: AT1G30380
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 13.2 | 8.5  kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Arachis hypogea, Brachypodium distachyon, Brassica napus, Cannabis sativa, Capsicum annuum, Dendrobium catenatum, Glycine max, Gossypium sp., Hordeum vulgare, Magnolia sinica, Malus domestica, Manihot esculenta, Medicago truncatula, Nicotania tabacum, Oryza sativa, Phragmites australis, Physcomitrium patens, Pisum sativum, Populus sp., Raphanus sativus, Ricinis communis, Solanum lycopercicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum sp., Vitis vinifera, Zea mays

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-PsaK antibodies
    1 - Chloroplast samples corresponding to 1.0 µg chlorophyll of Arabidopsis thaliana
    2 – Thylakoid membrane samples corresponding to 1.0 µg chlorophyll of Arabidopsis thaliana
    3 – Stromal fraction samples corresponding to 1.0 µg chlorophyll of Arabidopsis thaliana
    Mark: MW markers

    Protein samples corresponding to 1.0 µg chlorophyll of chloroplasts extracted from Arabidopsis thaliana.  Chloroplast samples were isolated with 20 mM Tricine–NaOH, pH 8.4, containing 400 mM sorbitol, 5 mM MgCl2, 5 mM MnCl2, 2 mM EDTA, 10 mM NaHCO3, 0.5% (w/v) bovine serum albumin (BSA) and 5 mM ascorbate: and denatured with exact buffer (62.5 mM Tris-HCl, pH 6.8, containing 2% (w/v) SDS, 10% (w/v) glycerol, 0.005% (w/v) bromophenol blue, and 50 mM dithiothreitol) at 95 °C/5 min.  Samples were separated at RT on 10%-20% gradient gel and blotted for 0.5 h to PVDF (pore size of  0.2  µm), using: semi-dry transfer at RT. Blot was blocked with 5 % milk  for: 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:  1,000 for 1h/RT with agitation in TBS-T. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in  matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera) diluted to 1: 25,000 in  for 1h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent: AS16 ECL-N-10 AgriseraBright (mid picogram). Exposure time was 3 minutes.

    Courtesy of Dr. Yuki Okegawa, Okayama University, Japan

  • Background
  • Background: PsaK is a subunit of photosystem I. It has a role in organizing the peripheral light-harvesting complexes on the core antenna of photosystem I. Alternative names: photosystem I subunit X, PSI-K
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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