Plastid acyl-ACP desaturase
AS13 2668 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
|Data sheet||Product citations||Protocols||Add review|
putative mature form of Chlamydomonas reinhardtii plastid acyl-ACP desaturase expressed in E.coli, UniProt: A8IQB8
40 | 35 kDa
Species of your interest not listed? Contact us
20 µg of soluble protein from Chlamydomonas reinhardii, were separated on 10 % SDS-PAGE and blotted for 1h to nitrocellulose. Blots were blocked with 1 % milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the plastid acyl-ACP desaturase antibody in 1 % milk in PBS-T at a dilution 1: 1500 for 2h at RT with agitation (left panel) or pre-immune serum (right panel). The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG, ALP conjugated) diluted to 1:3 000 in for 1h at RT with agitation. The blot was washed as above and reaction was visualized using BCIP/NBT.
Courtesy Dr. Dudley Page, UCLA, USA
Related products: Plastid acyl-ACP desaturase
This product can b...