A12,2 | RNA polymerase I subunit (homolog of Pol II Rpb9)

Product no: AS07 225

AS07 225 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

319
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    KLH-conjugated peptide derived from the Arabidopsis thaliana A12.2 (At3g25940) protein sequence. This sequence is only weakly conserved in other eukaryotic sequences available in the databases.

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 200 g
    Reconstitution: For reconstitution add 143 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000 (WB)
    Expected | apparent MW: 13,6 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity:

    Arabidopsis thaliana

    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    10 µg of total protein from (1) Arabidopsis thaliana and (4) Oryza sativa leafs extracted with PEB (AS08 300), as well as (2) cytosolic and (3) nuclear fractions of Arbaidopsis thaliana leafs were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-A12.2 (AS07 255, 1:1000, 1h) and secondary anti-rabbit (1:20 000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent reagent using a Fuji LAS-3000 CCD (300s, standard sensitivity). The target A12.2 is specifically detected only in the nuclear extract of Arabidopsis thaliana (3).

  • Additional Information
  • Additional information:

    This product has previously been labelled as anti-At3g25940 transcription factor S-II (TFIIS) domain-containing protein.

    Protocol for isolation of cytosolic and nuclear fractions can be found here.

    Additional information (application): This antibody is specific for A12,2 subunit of RNA polymerase I but NOT RNA polymerase II or IV from Arabidopsis thaliana
  • Background
  • Background:

    RNA polymerase I is a nuclear located DNA-dependent enzyme involved in RNA elongation and regulation of transcription. In yeast subunit A12.2 has been described as homologous to the Rpb9 subunit from polymerase II.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts



    Preparation of cytosolic and nuclear protein fractions


    1. Prepare protoplasts from 50 ml Arabidopsis thaliana cell culture according to the protocol of PEG transfection.
    2. Resuspend protoplasts in 10 ml GH buffer and keep the solution on ice for 10 min.
    GH buffer: 100mM glycine
    0.1% Hexylene glycol
    0.37M (4.7% w/v) saccharose
    0.3mM Spermine
    1.0mM Spermidine
    pH 8.3 with Ca(OH)2
    3. To release nuclei add Triton X100 to a final concentration of 0.1%. Pipetting gently up and down several
    times with a plastic pipette might be necessary to lyse cells.
    4. After 5min sediment nuclei by centrifugation at 1000 xg for 15 min at 4°C. Save supernatant as the
    cytoplasmic fraction. Wash the pelleted nuclei two times with GHT (GH+0.1% TX100) then finally
    resuspended in a suitable volume of extraction buffer + protease inhibitors.

    Courtesy Dr. Laszlo Bako, Umeå Plant Science Centre

  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
Info
img missing
AS16 ECL-N |  low pico to mid femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-N-10, 10 ml. Trial size limited to one per customer

AS16 ECL-N-100, 100 ml

AS16 ECL-N-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 13 €
img missing
AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 17 €
Close