A12,2 | RNA polymerase I subunit (homolog of Pol II Rpb9)

AS07 225 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

A12,2 | RNA polymerase I subunit (homolog of Pol II Rpb9) in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS07 225)
A12,2 | RNA polymerase I subunit (homolog of Pol II Rpb9)


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Product Information


KLH-conjugated peptide derived from the Arabidopsis thaliana A12.2 (At3g25940) protein sequence. This sequence is only weakly conserved in other eukaryotic sequences available in the databases.

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 143 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW 13,6 kDa


Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application example

10 µg of total protein from (1) Arabidopsis thaliana and (4) Oryza sativa leafs extracted with PEB (AS08 300), as well as (2) cytosolic and (3) nuclear fractions of Arbaidopsis thaliana leafs were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-A12.2 (AS07 255, 1:1000, 1h) and secondary anti-rabbit (1:20 000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent reagent using a Fuji LAS-3000 CCD (300s, standard sensitivity). The target A12.2 is specifically detected only in the nuclear extract of Arabidopsis thaliana (3).

Additional information

Additional information

This product has previously been labelled as anti-At3g25940 transcription factor S-II (TFIIS) domain-containing protein.

Protocol for isolation of cytosolic and nuclear fractions can be found here.

This antibody is specific for A12,2 subunit of RNA polymerase I but NOT RNA polymerase II or IV from Arabidopsis thaliana

Related products



RNA polymerase I is a nuclear located DNA-dependent enzyme involved in RNA elongation and regulation of transcription. In yeast subunit A12.2 has been described as homologous to the Rpb9 subunit from polymerase II.

Product citations

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