His-tag | 6xHis, conjugated to SureLight® R-Phycoerythrin (Clone AD1,1,10)

AS15 2931 | Clonality: monoclonal | Host: Mouse | Reactivity: Polyhisitidine tagged proteins (N- or C-terminus label)

Product Information

Immunogen Polyhistidine (HHHHHH) tagged polypeptide

Host Mouse

Clonality Monoclonal

Purity Immunogen affinity purified in 100 mM Sodium Phosphate (pH 7.4), 100 mM NaCl, 50 mM Sucrose, 1.5% BSA and 0.01% Thimerosal.

Format Lyophilized

Quantity 100 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.

Tested applications Flow cytometry (Flow cyt), Cell-based assays (CBA), Microarrays (MA), and Microplate applications (MPl)

Recommended dilution The optimal working dilution should be determined by the investigator

Reactivity

Application examples

Additional information

Additional information Antibody is provided in: 100 mM Sodium Phosphate (pH 7,4), 100 mM Sodium Chloride, 50 mM Sucrose, 0,1% BSA and 2 mM Sodium Azide

Background

Background Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to SureLight® R-Phycoerythrin. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.