95 kDa Lcm (phycobilisome - to- thylakoid core linker with phycocyanobilin chromophore)

Product no: AS08 281

AS08 281  |  Clonality:Polyclonal  |  Host:Rabbit  |  Reactivity:Cyanobacteria and red algae

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  • Product Info
  • Immunogen:

    native protein purified from phycobilisomes of Porphyridium cruentum

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 200 l
    Reconstitution: For reconstitution add 200 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 300-1 : 1000 (WB)
    Expected | apparent MW:

    95 kDa (Porphyridium cruentum), 75-120 kDa (other species)

  • Reactivity
  • Confirmed reactivity: Anabaena variablis, Aphanathece halophytica, Fremyella diplosiphon (Microchaete diplosiphon CCALA 811), Mastigocladus laminosus, Nostoc sp., Synechococcus sp. PCC 7002, ATCC 27264, PR6) , Synechococcus elongatus (strain PCC 7942), Anacystis nidulans R2), Synechococcus leopolensis (..elangatus PCC 6301), Synechecoccus lividus, Spirulina platensis (Athrospira platensis), Tolypothrix tenuis; red alga: Porphyridium cruentum (purpureum), P. aeruginuso, Rhodosorus marinus, Griffithsia monilis
    Predicted reactivity: Algae (red), Cyanobacteria
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    Anabaena sp. sample can serve as a negative control, as under nitrogen deficient conditions phycobiliproteins are going to be lost.
    Overall sample quality is of crucial importance and in older or not properly stored samples, phycobiliproteins will undergo proteolytic degradation.

  • Background
  • Background:

    A large polypeptide Lcm with a few phycocyanobilin chromophores which acts as a terminal energy acceptor and as a linker polypeptide between the phycobilisomes and the photosynthetic reaction centres (PS2).

  • Product Citations
  • Selected references: Redlinger & Gantt (1982) A Mr 95,000 polypeptide in Porphyridium cruentum phycobilisomes and thylakoids: PNAS 79:5542.
    Redlinger & Gantt (1981). Phycobilisome structure of Porphyridium cruentum. Plant Physiol. 68:1375.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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