95 kDa Lcm (phycobilisome - to- thylakoid core linker with phycocyanobilin chromophore)
AS08 281 | Clonality:Polyclonal | Host:Rabbit | Reactivity:Cyanobacteria and red algae
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Product Information
Immunogen
native protein purified from phycobilisomes of Porphyridium cruentum
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
200 µl
Reconstitution
For reconstitution add 200 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 300-1 : 1000 (WB)
Expected | apparent MW
95 kDa (Porphyridium cruentum), 75-120 kDa (other species)
Reactivity
Confirmed reactivity
Anabaena variablis, Aphanathece halophytica, Fremyella diplosiphon (Microchaete diplosiphon CCALA 811), Mastigocladus laminosus, Nostoc sp., Synechococcus sp. PCC 7002, ATCC 27264, PR6) , Synechococcus elongatus (strain PCC 7942), Anacystis nidulans R2), Synechococcus leopolensis (..elangatus PCC 6301), Synechecoccus lividus, Spirulina platensis (Athrospira platensis), Tolypothrix tenuis; red alga: Porphyridium cruentum (purpureum), P. aeruginuso, Rhodosorus marinus, Griffithsia monilis
Predicted reactivity
Algae (red), Cyanobacteria
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Anabaena sp. sample can serve as a negative control, as under nitrogen deficient conditions phycobiliproteins are going to be lost.
Overall sample quality is of crucial importance and in older or not properly stored samples, phycobiliproteins will undergo proteolytic degradation.
Background
Background
A large polypeptide Lcm with a few phycocyanobilin chromophores which acts as a terminal energy acceptor and as a linker polypeptide between the phycobilisomes and the photosynthetic reaction centres (PS2).
Product citations
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