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ACC synthase 8 | 1-aminocyclopropane-1-carboxylate synthase 8

AS06 202 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of thylakoid lumen

ACC synthase 8 | 1-aminocyclopropane-1-carboxylate synthase 8  in the group Antibodies for Plant/Algal  / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS19 4350)

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Product Information

Immunogen Recombinant Arabidopsis thaliana 1-aminocyclopropane-1-carboxylate synthase 8 protein, amino acids: 1-469, Uniprot: Q9T065, TAIR: AT4G37770
Host Rabbit
Clonality Polyclonal
Purity >95%, Protein G purified, total immunoglobulin G fraction
Format Liquid
Quantity 50 ul
Storage Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 53 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example


Western blot using anti-ACS8 antibodies


80 and 40 µg of recombinant ACS8 protein were separated on  8 % SDS-PAGE  and blotted 1h to PVDF. Blot was blocked with 5 % milk for 2h/RT in PBS-T with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in PBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in  for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions. 

Additional information

Additional information Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4
Reactivity of this antibody on endogenous protein remains to be determined.

Related products

Related products AS11 1800 | Anti-ACC | 1-Aminocyclopropane-1-carboxylic acid, rabbit antibodies
AS14 2774 | Anti-ACC synthase 7 | 1-aminocyclopropane-1-carboxylate synthase 7, rabbit antibodies

Background

Background ACC synthase 8 belongs to a group fo enzymes which catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene. ACS8 is auxin inducible. 
Alternative names:
1-aminocyclopropane-1-carboxylate synthase 8, ACC synthase 8, S-adenosyl-L-methionine methylthioadenosine-lyase 8.

Product citations

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