ACD | alpha crystalline domain of sHSP

Product no: AS15 3029

AS15 3029  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: recombinant ACD domain od sHSP

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from sequences of ACD domain
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 16,6 kDa
  • Reactivity
  • Confirmed reactivity: recombinant sHSP with ACD domain
    Predicted reactivity: ACD domain of sHSP
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

     

     

     Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis  PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody  (1: 10 000).

     

     Western blot using anti-Hsp16.6 antibodies
  • Background
  • Background: ACD (alpha crystalline domain)
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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
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