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ACD | alpha crystalline domain of sHSP

AS15 3029  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: recombinant ACD domain od sHSP

ACD | alpha crystalline domain of sHSP in the group Antibodies Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS15 3029)
ACD | alpha crystalline domain of sHSP



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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen KLH-conjugated peptide derived from sequences of ACD domain
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 16,6 kDa

Reactivity

Confirmed reactivity recombinant sHSP with ACD domain
Predicted reactivity ACD domain of sHSP
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application example

 

 

 Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis  PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody  (1: 10 000).

 

 Western blot using anti-Hsp16.6 antibodies

Additional information

Related products

Related products

AS08 350 | Anti-DnaK2 | heat shock protein 70-2 (HSP70-2), rabbit antibodies

collection of antibodies to heat shock proteins

Background

Background ACD (alpha crystalline domain)

Product citations

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