ACD | alpha crystalline domain of sHSP

AS15 3029 | Clonality: Polyclonal | Host: Rabbit | Reactivity: recombinant ACD domain od sHSP

Product Information

Immunogen KLH-conjugated peptide derived from sequences of ACD domain

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 16,6 kDa

Reactivity

Confirmed reactivity recombinant sHSP with ACD domain

Predicted reactivity ACD domain of sHSP

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example

Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000).