ACD | alpha crystalline domain of sHSP
AS15 3029 | Clonality: Polyclonal | Host: Rabbit | Reactivity: recombinant ACD domain od sHSP

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Product Information
Immunogen
KLH-conjugated peptide derived from sequences of ACD domain
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
16.6 kDa
Reactivity
Confirmed reactivity
recombinant sHSP with ACD domain
Predicted reactivity
ACD domain of sHSP
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
Application example
Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000). |
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Additional information
Background
Background
ACD (alpha crystalline domain)
Product citations
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