ACT | Actin (monoclonal, clone mAbGPa 10-B3)
AS16 3141 | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Dictyostelium discoideum

Data sheet | Product citations | Add review |
Product Information
Immunogen
Full length recombinant Arabidopsis thaliana Actin-8 (ACT8) expressed in E.coli, Uniprot: Q96293
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG2b
Purity
Immunoglobulin IgG2b, Protein G purified in 0.1M Sodium Phosphate, pH 7.4. Contains 0.15M NaCl, 0.05% (w/v) sodium azide.
Format
Liquid
Quantity
100 ĩg
Storage
Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1-2 ug/ml (WB)
Expected | apparent MW
45 | 45 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Dictyostelium discoideum
Predicted reactivity
Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Camelina sativa, Camellia lipoensis, Cucumis sativus, Dendrocalamus latiflorus, Dionaea muscipula, Gossypium sp., Eucalyptus grandis, Euphorbia lathyris, Gynura bicolor, Ficus microcarpa, Haloxylon ammodendron, Helianthus annuus, Hevea brasiliensis, Lilium regale, Medicago sativa, Mimosa pudica, Nitraria sibirica, Oryza sativa subsp. japonica, Oryza sativa subsp. indica, Oxytropis ochrocephala, Paeonia lactiflora, Panax notoginseng, Populus trichocarpa, Prunus avium, Ricinus communis, Striga asiatica, Theobroma cacao, Torenia fournieri, Trifolium pRatense, Ziziphus jujuba
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
This antibody is purified on a protein G column. It recognizes Arabidopsis actins ACT1, 2, 3, 4, 7, 8, 11, 12 and Dictyostelium actin
Background
Background
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Product citations
Selected references
Sultan et al. (2017). The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria. Plant Cell. 2016 Nov;28(11):2805-2829.
Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281
Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281
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