ACT | Actin (monoclonal, clone mAbGPa 10-B3)

Name: ACT | Actin (monoclonal, clone mAbGPa 10-B3)
Brand: IKP
SKU: AS16 3141
Price: 387 EUR
Availability: InStock

AS16 3141 | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Dictyostelium discoideum

ACT | Actin (monoclonal, clone mAbGPa 10-B3) in the group Antibodies Plant/Algal / Plant Developmental Biology / Cytoskeleton at Agrisera AB (Antibodies for research) (AS16 3141)

ACT | Actin (monoclonal, clone mAbGPa 10-B3)

Product Information

Immunogen Full length recombinant Arabidopsis thaliana Actin-8 (ACT8) expressed in E.coli, Uniprot: Q96293

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG2b

Purity Immunoglobulin IgG2b, Protein G purified in 0.1M Sodium Phosphate, pH 7.4. Contains 0.15M NaCl, 0.05% (w/v) sodium azide.

Format Liquid

Quantity 100 µg

Storage Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1-2 ug/ml (WB)

Expected | apparent MW

45 | 45 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Dictyostelium discoideum

Predicted reactivity Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Camelina sativa, Camellia lipoensis, Cucumis sativus, Dendrocalamus latiflorus, Dionaea muscipula, Gossypium sp., Eucalyptus grandis, Euphorbia lathyris, Gynura bicolor, Ficus microcarpa, Haloxylon ammodendron, Helianthus annuus, Hevea brasiliensis, Lilium regale, Medicago sativa, Mimosa pudica, Nitraria sibirica, Oryza sativa subsp. japonica, Oryza sativa subsp. indica, Oxytropis ochrocephala, Paeonia lactiflora, Panax notoginseng, Populus trichocarpa, Prunus avium, Ricinus communis, Striga asiatica, Theobroma cacao, Torenia fournieri, Trifolium pRatense, Ziziphus jujuba
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

This antibody is purified on a protein G column. It recognizes Arabidopsis actins ACT1, 2, 3, 4, 7, 8, 11, 12 and Dictyostelium actin

Background

Background Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

Product citations

Selected references Sultan et al. (2017). The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria. Plant Cell. 2016 Nov;28(11):2805-2829.
Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281

immunogen:	Full length recombinant Arabidopsis thaliana Actin-8 (ACT8) expressed in E.coli, Uniprot: Q96293
Sub class:	IgG2b
Host:	Mouse
Clonality:	Monoclonal
Purity:	Immunoglobulin IgG2b, Protein G purified in 0.1M Sodium Phosphate, pH 7.4. Contains 0.15M NaCl, 0.05% (w/v) sodium azide.
Format:	Liquid
Quantity:	100 µg
storage:	Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	1-2 ug/ml (WB)
calculated \| apparent molecular mass [kDa]:	45 \| 45 kDa
Confirmed reactivity:	Arabidopsis thaliana, Dictyostelium discoideum
predicted reactivity:	Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Camelina sativa, Camellia lipoensis, Cucumis sativus, Dendrocalamus latiflorus, Dionaea muscipula, Gossypium sp., Eucalyptus grandis, Euphorbia lathyris, Gynura bicolor, Ficus microcarpa, Haloxylon ammodendron, Helianthus annuus, Hevea brasiliensis, Lilium regale, Medicago sativa, Mimosa pudica, Nitraria sibirica, Oryza sativa subsp. japonica, Oryza sativa subsp. indica, Oxytropis ochrocephala, Paeonia lactiflora, Panax notoginseng, Populus trichocarpa, Prunus avium, Ricinus communis, Striga asiatica, Theobroma cacao, Torenia fournieri, Trifolium pRatense, Ziziphus jujuba Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known.
additional information:	This antibody is purified on a protein G column. It recognizes Arabidopsis actins ACT1, 2, 3, 4, 7, 8, 11, 12 and Dictyostelium actin
background:	Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
All references:	Sultan et al. (2017). The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria. Plant Cell. 2016 Nov;28(11):2805-2829. Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281