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Anti-HSV epitope tag
Product no: AS21 4603
AS21 4603 | Clonality: Polyclonal | Host: Rabbit | Reactivity: HSV-tagged fusion protein
329
€
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Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated peptide QPELAPEDPED Host: Rabbit
Clonality: Polyclonal
Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: depends upon fusion partner - Reactivity
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Confirmed reactivity: HSV-tagged fusion proteins - Application Examples
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N-terminal tagged HSV-GFP expressed in E. coli is recognized by the anti HSV AS21 4603 antibody. E. coli strains (DH5alpha, NEB) transformed with the HSV-GFP plasmid or with an empty plasmid (neg. control) were grown overnight in LB media supplemented with the appropriate antibiotic. Cells were pelleted and resuspended in 1X Laemmli loading buffer. Cells were boiled for 1 min at 100°C and the extract clarified by centrifugation. Total extracts were then separated on a 12% acrylamide gel, which was transferred on a 0,1 µm nitrocellulose membrane. The Ponceau-stained membrane is presented on the left. The membrane was blocked 1h in PBS-tween 0,1% with nonfat milk at 5%. The anti-HSV antibody (AS21 4603) was incubated overnight at 4°C at a 1:5000 dilution. The secondary antibody (anti-Rabbit, HRP-conjugated was used at 1:10 000 dilution) was further incubated 3 h at room temperature. Detection was performed with the ChemiDoc Touch with the chemiluminescent detection reagent, according to manufacture's recommendations. The image (right panel) was obtained after 57 s exposure in optimal auto exposure mode.
Courtesy of Lisa Westrich, Mathieu Jacquemin, Katia Wostrikoff and Olivier Vallon - Background
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Background: The HSV tag consists of a short peptide sequence which is 11 amino acids long, derived from the herpes simplex virus (HSV) envelope glycoprotein D. Fusion of a protein of interes to HSV tag allows for target protein detection and analysis using immunochemical methods, such as western blotting, immunoprecipitation, and immunocytochemistry. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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