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Anti-MLH1 | DNA mismatch repair protein MLH1
AS24 5042| Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated peptide derived from, Arabidopsis thaliana MLH1 protein, UniProt: Q9ZRV4
TAIR: AT4G09140Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Immunofluorescence (IF) Recommended dilution: 1_100 - 1: 200 (IF) Expected | apparent MW: 83.4 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Arachis hypogaea, Brachypodium distachyon, Brassica napus, Cannabis sativa, Capsicum annuum, Citrus sp., Cucumis sativus, Glycine max, Gossypium sp., Hordeum vulgare, Malus domestica, Manihot esculenta, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Populus sp., Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum sp., Vitis vinifera, Zea mays
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Type of material: meiocytes of Arabidopsis thaliana
Fixation: formaldehyde
Hydrophilization: no
Cell wall digestion: yes
Membrane permeabilization: PBS1X- Triton 1%- EDTA 1mM
Antigen retrieval: no Blocking buffer: 3% BSAxPBS1X -Tween 0.1%
Washing buffer: PBS1X -Tween 0.1%
Primary antibody dilution and incubation time: 1:200 72 h
Secondary antibody dilution and incubation time and supplier: Star green 1:250 abberior
Co-staining of the nucleus (DAPI): yes
Cell wall and nucleus staining: no
Courtesy of Dr. Stephanie Durand, Max Planck Institute for Plant Breeding Research (MPIPZ), Germany
- Background
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Background: MLH1 is a key component of the DNA mismatch repair (MMR) system, which is crucial for maintaining genomic stability. It helps correct DNA replication errors and plays an essential role in meiosis, ensuring proper chromosome segregation and genetic diversity. Alternative names: MutL protein homolog 1, Protein MUTL-HOMOLOGUE 1 (AtMLH1)
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
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