Anti-P2K1| L-type lectin-domain containing receptor kinase I.9

Product no: AS22 4826

AS22 4826 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana recombinant P2K1
 

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana DORN1 protein, UniProt: Q9LSR8-1, TAIR: AT5G60300
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immonogen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 85 | 100-110 kDa (due to heavy glycosylation)
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana recombinant P2K1
    Not reactive in: Musa paradisiaca, Piper sp., Zingiber officinale
  • Additional Information
  • Additional information (application): P2K1 protein is of low abundance, according to Protein Abundance Database, PAX. Therefore, to detect P2K1 in endogenous sample, high protein load of minimum 50 µg/well of membrane fraction and extreme low femtogram detection range sensitivity of detection reagent must be applied.
  • Background
  • Background: P2K1 (L-type lectin-domain containing receptor kinase I.9) is an essential receptor for extracellular ATP. It binds ATP with high affinity through its extracellular legume-lectin like region and is  required for ATP-induced intracellular calcium response, mitogen-activated protein kinase 3 (MPK3) and MPK6 activation and ATP-induced gene expressio. 

    Alternative name: Protein DOES NOT RESPOND TO NUCLEOTIDES 1, Purinoreceptor kinase 1.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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