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COI1 | Coronate insensitive 1 (rabbit antibody)

AS12 2637 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

COI1 | Coronate insensitive 1 (rabbit antibody) in the group Antibodies Plant/Algal  / Plant Developmental Biology / Plant Signal Transduction at Agrisera AB (Antibodies for research) (AS12 2637)



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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana COI1 protein, UniProt: O04197 , TAIR: At2g39940

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 25 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

70 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica rapa, Glycine max, Lupinus luteus, Medicago tribuloides, Nicotiana tabacum, Oryza sativa, Pisum sativum, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Total proteins were isolated from 7-day old Arabidopsis thaliana Col-0 (wild-type) and coi1 (null mutant) seedlings according to [ref 1]. Proteins were denatured at 65°C for 5 min. 30 μg of proteins were separated on a 10% SDS-PAGE and transferred for 70 min at 55V using a tank transfer system to nitrocellulose membrane. Blots were blocked with 1x PBS + 0.1% Tween 20 (PBS- T) + 5% milk for 1hr at room temperature (RT) with agitation. Blots were incubated in primary ⍺-COI1 antibody (AS12 2637) diluted to 1:5,000 in PBS-T+5 % milk at 4°C overnight with agitation. The primary antibody solution was decanted, and the blots were washed four times (6-8 minutes each) in 1x PBS-T at RT with agitation prior to incubation with secondary antibody Goat anti Rabbit IgG (H&L) – HRP conjugated (AS09 602-trial) diluted to 1:20,000 in 1x PBS-T + 5% milk for 2hr at RT with agitation. The blots were washed as above and developed for 4 min with chemiluminescent detection reagent ECL Bright (AS16 ECL-N). Exposure times (in seconds) to X-ray films were done as indicated. PonceauS stained membrane and membrane portion probed with ⍺-MPK6 served as loading controls [ref 1].

Courtesy of Alani Antoine-Mitchell and Antje Heese, University of Missouri, Div. Biochemistry, IPG (USA)

References: [1] LaMontagne, ED., Collins, CA., Peck, S.C., and Heese, A. 2016. Isolation of microsomal membrane proteins from Arabidopsis thaliana. Curr. Protoc. Plant Biol. 1:217-234. doi: 10.1002/cppb.20020

Additional information

Antibody detects 100 ng of recombinant COI1 protein, expressed in Hi5 suspenstion insect cells. 

The peptide used to elicit this antibody is partially conserved in Zea mays: ZmCOI1a, ZMCOI1b, ZMCO1c and ZmCOI2. 

Related products

Background

Background COI1 (Coronate insensitive 1) is an esential component of jasmonate receptor. Required for jasmonate-regulated plant fertility and defense processes, and for coronatine and/or other elicitors perceptions/responses. Alternative names: F-box/LRR-repeat protein 2, AtCOI1, AtFBL2.

Product citations

Selected references Agrawal et al. (2022) MEDIATOR SUBUNIT17 integrates jasmonate and auxin signaling pathways to regulate thermomorphogenesis. Plant Physiol. 2022 Aug 1;189(4):2259-2280. doi: 10.1093/plphys/kiac220. PMID: 35567489.
immunogen:

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana COI1 protein, UniProt: O04197 , TAIR: At2g39940

Reconstitution: For reconstitution add 25 µl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Quantity: 50 µg
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
recommended dilution: 1 : 1000 (WB)
calculated | apparent molecular mass [kDa]:

70 kDa

Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Brassica rapa, Glycine max, Lupinus luteus, Medicago tribuloides, Nicotiana tabacum, Oryza sativa, Pisum sativum, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Vitis vinifera
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Picture (footer):

Total proteins were isolated from 7-day old Arabidopsis thaliana Col-0 (wild-type) and coi1 (null mutant) seedlings according to [ref 1]. Proteins were denatured at 65°C for 5 min. 30 μg of proteins were separated on a 10% SDS-PAGE and transferred for 70 min at 55V using a tank transfer system to nitrocellulose membrane. Blots were blocked with 1x PBS + 0.1% Tween 20 (PBS- T) + 5% milk for 1hr at room temperature (RT) with agitation. Blots were incubated in primary ⍺-COI1 antibody (AS12 2637) diluted to 1:5,000 in PBS-T+5 % milk at 4°C overnight with agitation. The primary antibody solution was decanted, and the blots were washed four times (6-8 minutes each) in 1x PBS-T at RT with agitation prior to incubation with secondary antibody Goat anti Rabbit IgG (H&L) – HRP conjugated (AS09 602-trial) diluted to 1:20,000 in 1x PBS-T + 5% milk for 2hr at RT with agitation. The blots were washed as above and developed for 4 min with chemiluminescent detection reagent ECL Bright (AS16 ECL-N). Exposure times (in seconds) to X-ray films were done as indicated. PonceauS stained membrane and membrane portion probed with ⍺-MPK6 served as loading controls [ref 1].

Courtesy of Alani Antoine-Mitchell and Antje Heese, University of Missouri, Div. Biochemistry, IPG (USA)

References: [1] LaMontagne, ED., Collins, CA., Peck, S.C., and Heese, A. 2016. Isolation of microsomal membrane proteins from Arabidopsis thaliana. Curr. Protoc. Plant Biol. 1:217-234. doi: 10.1002/cppb.20020
additional information (application):

Antibody detects 100 ng of recombinant COI1 protein, expressed in Hi5 suspenstion insect cells. 

The peptide used to elicit this antibody is partially conserved in Zea mays: ZmCOI1a, ZMCOI1b, ZMCO1c and ZmCOI2. 

background: COI1 (Coronate insensitive 1) is an esential component of jasmonate receptor. Required for jasmonate-regulated plant fertility and defense processes, and for coronatine and/or other elicitors perceptions/responses. Alternative names: F-box/LRR-repeat protein 2, AtCOI1, AtFBL2.
All references: Agrawal et al. (2022) MEDIATOR SUBUNIT17 integrates jasmonate and auxin signaling pathways to regulate thermomorphogenesis. Plant Physiol. 2022 Aug 1;189(4):2259-2280. doi: 10.1093/plphys/kiac220. PMID: 35567489.

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