COI1 | Coronate insensitive 1 (rabbit antibody)
AS12 2637 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Samples are 10-day old Arabidopsis thaliana seedlings of Col-0 and coi1-1 mutant (premature stop codon at W467* vs a full length protein of 592 aa), roots from 10-day old seedlings, either mock treated or treated with 20 uM MeJA. 20 ug of protein were separated on 4-12% NuPAGE gel and transferred onto PVDF membrane. The membrane was blocked with 5% skim milk dissolved in TBST. Primary antibody was added at a dilution of 1:1000 and incubated overnight at 4°C. The membrane was then washed 3X15 min with TBST at RT. Secondary antibody was added at a dilution of 1:10 000 (AS09 602, Agrisera) and incubated at RT for 1 hr, followed by washes for 3X15 min with TBST. The detection was performed using the ECL kit (SuperSignal West Pico from Thermo scientific).
Courtesy of Dr. Xuecheng Zhang, Richard B. Simches Research Center, USA
Antibody detects 100 ng of recombinant COI1 protein, expressed in Hi5 suspenstion insect cells.