COI1 | Coronate insensitive 1 (rabbit antibody)
AS12 2637 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana COI1 protein, UniProt: O04197 , TAIR: At2g39940
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
70 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Brassica rapa, Glycine max, Lupinus luteus, Medicago tribuloides, Nicotiana tabacum, Oryza sativa, Pisum sativum, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Vitis vinifera
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example
Samples are 10-day old Arabidopsis thaliana seedlings of Col-0 and coi1-1 mutant (premature stop codon at W467* vs a full length protein of 592 aa), roots from 10-day old seedlings, either mock treated or treated with 20 uM MeJA. 20 ug of protein were separated on 4-12% NuPAGE gel and transferred onto PVDF membrane. The membrane was blocked with 5% skim milk dissolved in TBST. Primary antibody was added at a dilution of 1:1000 and incubated overnight at 4°C. The membrane was then washed 3X15 min with TBST at RT. Secondary antibody was added at a dilution of 1:10 000 (AS09 602, Agrisera) and incubated at RT for 1 hr, followed by washes for 3X15 min with TBST. The detection was performed using the ECL kit (SuperSignal West Pico from Thermo scientific).
Courtesy of Dr. Xuecheng Zhang, Richard B. Simches Research Center, USA
Samples are 10-day old Arabidopsis thaliana seedlings of Col-0 and coi1-1 mutant (premature stop codon at W467* vs a full length protein of 592 aa), roots from 10-day old seedlings, either mock treated or treated with 20 uM MeJA. 20 ug of protein were separated on 4-12% NuPAGE gel and transferred onto PVDF membrane. The membrane was blocked with 5% skim milk dissolved in TBST. Primary antibody was added at a dilution of 1:1000 and incubated overnight at 4°C. The membrane was then washed 3X15 min with TBST at RT. Secondary antibody was added at a dilution of 1:10 000 (AS09 602, Agrisera) and incubated at RT for 1 hr, followed by washes for 3X15 min with TBST. The detection was performed using the ECL kit (SuperSignal West Pico from Thermo scientific).
Courtesy of Dr. Xuecheng Zhang, Richard B. Simches Research Center, USA
Additional information
Antibody detects 100 ng of recombinant COI1 protein, expressed in Hi5 suspenstion insect cells.
The peptide used to elicit this antibody is partially conserved in Zea mays: ZmCOI1a, ZMCOI1b, ZMCO1c and ZmCOI2.
Background
Background
COI1 (Coronate insensitive 1) is an esential component of jasmonate receptor. Required for jasmonate-regulated plant fertility and defense processes, and for coronatine and/or other elicitors perceptions/responses. Alternative names: F-box/LRR-repeat protein 2, AtCOI1, AtFBL2.
Product citations
Selected references
Agrawal et al. (2022) MEDIATOR SUBUNIT17 integrates jasmonate and auxin signaling pathways to regulate thermomorphogenesis. Plant Physiol. 2022 Aug 1;189(4):2259-2280. doi: 10.1093/plphys/kiac220. PMID: 35567489.
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