Anti-WRKY33 | Probable WRKY transcription factor 33

Product no: AS22 4880

AS22 4880 | Clonality: Polyclonal|  Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from WRKY33 of Arabidopsis thaliana, UniProt: Q8S8P5 TAIR: AT2G38470
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000 (WB)
    Expected | apparent MW: 57 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brassica napus

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-WRKY33 antibodies

    Samples:
    1 - 50 µg of Arabidopsis thaliana whole leaf extract
    2 - 50 µg of Arabidopsis thaliana wrky33-2 mutant

    50 µg/well of total protein extracted freshly from Arabidopsis thaliana. Exact buffer components were: 6% glycerol, 2% SDS, 50mM Tris-HCl pH6.8, 0.004% Bromophenol blue and 1% β-ME. Samples were denatured at 100 °C for 5 min, cooled down on ice, and were separated on 10% SDS-PAGE and blotted for 30 min/RT to PVDF (pore size of 0.2 µm), using semi-dry transfer. Blot was blocked with 5% milk 1 h/RT. Blot was incubated in the primary antibody at a dilution of 1: 2000 ON/4°C with agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 10 000 in 5% milk for 1h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent. Exposure time was 30 seconds.

    Courtesy of Dr. Jinggeng Zhou, Shanghai Normal University, China

  • Background
  • Background: WRKY33 (Probable WRKY transcription factor 33 ) functions with WRKY25 and WRKY26 as positive regulator of plant thermotolerance. Involved in defense responses. Required for pathogen resistance.

    Alternative name: WRKY DNA-binding protein 33

  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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