AS19 4336 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Nicotiana tabacum
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Varied amounts of recombinant Nicotiana tabacum osmotin were loaded/well and separated on 10 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at 3 µg/ml for 1h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions.
Reactivity of this antibody on endogenous sample remains to be determined.