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Osmotin

AS19 4336 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Nicotiana tabacum

Osmotin in the group Antibodies for Plant/Algal  / Environmental Stress / Drought stress at Agrisera AB (Antibodies for research) (AS19 4336)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen Recombinant Osmotin derived from Nicotiana tabacum protein sequence, amino acids: 22-246. UniProt: P14170

Host Rabbit
Clonality Polyclonal
Purity >95%, Protein G purified, total immunoglobulin G fraction
Format Liquid
Quantity 50ug
Storage Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 500 - 1: 1000 (WB)
Expected | apparent MW 26 | 27 kDa

Reactivity

Confirmed reactivity Nicotiana tabacum
Predicted reactivity Capsicum annuum, Solanum lycopersicum
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example


western blot using anti-osmotin antibodies


Varied amounts of recombinant Nicotiana tabacum osmotin were loaded/well and separated on 10 % SDS-PAGE  and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at 3 µg/ml for 1h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in  for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions. 

Additional information

Additional information Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4

Reactivity of this antibody on endogenous sample remains to be determined.

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Background

Background Osmotin protein (from thaumatin protein family), coded by a gene AP24. Known to inhibit the germination and growth of the fungus Phytophthora infestans. Protein is induced by: salt stress, ABA viral infection and wounding. Localised to vacuole.

Product citations

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