SAL1 | Sal1 phosphatase

Recombinant SAL1, full-length protein, 353 amino acids. The cDNA of SAL1 (At5g63980, protein Q42546) was cloned into pHUE expression vector and the protein has been produced and purified according to Baker et al 2005 

37.5 | 41 kDa (Arabidopsis thaliana)

Chlamydomonas reinhardii

application example

7.5 µg of total leaf protein extracted with PEB (AS08 300) from (1) Nicotiana tabacum, (2) Glycine max, (3) Lycopersicon esculentum, (4) Chlamydomonas reinhardtii, (5) Populus tremula and (6) Arabidopsis thaliana were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SAL1 (AS07 256, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) HRP-conjugated antibody in TBS-T containing 2% low fat milk powder.  Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent,  using a Fuji LAS-3000 CCD (240s, standard sensitivity).

The plant SAL1 (known also as FIERY1, FRY1, HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 2, HOS2, MBM17.8, MBM17_8) is a 353 aa protein homologous to the HAL2 and CysQ phosphatases of yeast and Escherichia coli, respectively. The SAL1 protein expressed in E. coli shows nucleotidase and inositol phosphatase activities. SAL1 is proposed to participate in the sulfur assimilation pathway as well as in the phosphoinositide signaling pathway.