Anti-APC | allophycocyanin alpha and beta

Product no: AS08 277

AS08 277  |  Clonality:Polyclonal  |  Host:Rabbit  |  Reactivity: Red algae, cyanobacteria

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  • Product Info
  • Immunogen:

    native allophycocyanin alpha and beta purified from Porphyridium cruentum phycobilisomes

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunolocalization (IL), Western blot (WB)
    Recommended dilution: 1 : 200-1 : 500 (IL), 1 : 1500 - 3000 (WB)
    Expected | apparent MW:

    14-19 kDa

  • Reactivity
  • Confirmed reactivity: Porphyridium cruentum, Synechocystis PCC 6803
    Predicted reactivity: Red Algae, Cyanobacteria
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application examples:

    Reactant: Synechocystis

    Application: Western Blotting

    Pudmed ID: 24476911

    Journal: Nucleic Acids Res

    Figure Number: 6C

    Published Date: 2014-04-01

    First Author: Gunnelius, L., Hakkila, K., et al.

    Impact Factor: 16.476

    Open Publication

    Photosynthetic properties of ?rpoZ. (A) Light-saturated photosynthetic activity of a 1 ml of culture (OD730 = 1) of CS (white bar) and ?rpoZ (black bar) in standard conditions. Each bar represents an average of three independent biological replicates, and the error bars denote SE. (B) Fluorescence at 77 K was measured using 440-nm light that excites Chl. The data were normalized by dividing with the height of the PSI emission peak at 723 nm. (C) Total proteins were isolated, separated with SDS-PAGE and the amounts of PSI reaction center protein PsaB, PSII core protein CP43 and the phycobilisome proteins allophycocyanin (APC) and phycocyanin (PC) were measured by western blotting. The protein contents of PsaB, CP43, ACP and PC were 99 ± 6%, 97 ± 7, 103 ± 7% and 99 ± 4, respectively, in ?rpoZ of that measured in CS.

  • Additional Information
  • Additional information: This product can be sold containing proclin if requested
  • Background
  • Background:

    Allophycocyanin is a protein of the light-harvesting phycobiliprotein family of red algae and cyanobacteria. It is an accessory pigment to chlorophyll A located in the core of the phycobilisome, and its strong spectral overlap with chlorophyll facilitates energy transfer for photosynthesis.

  • Product Citations
  • Selected references: Kurkela et al. (2017). Acclimation to High CO2 Requires the ω Subunit of the RNA Polymerase in Synechocystis. Plant Physiol. 2017 May;174(1):172-184. doi: 10.1104/pp.16.01953. Epub 2017 Mar 28.
    Ge at al. (2017). Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017 Jul;16(7):1258-1274. doi: 10.1074/mcp.M116.068080.
    Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.
    Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.
    Hernandez-Prieto et al. (2011). The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803: Their involvement in chlorophyll biogenesis for Photosystem II. Bioch.Bioph. Acta.
    Gantt & Lipschultz (1974). Phycobilisome structure by immuno-electron microscopy. J. Phycology, Vol. 13:3, pages: 185-192. (immunolocalization)
    Gantt & Lipschultz (1974). Phycobilisomes of Porphyridium cruentum: Pigment Analysis. Biochem. 13:2960. Gantt E & C Lipschultz (1977). Probing phycobilisome structure by immuno-electron microscopy. J Phycol. 13:18
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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