APC | allophycocyanin alpha and beta
AS08 277 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Red algae, cyanobacteria
|Data sheet||Product citations||Protocols||Add review|
native allophycocyanin alpha and beta purified from Porphyridium cruentum phycobilisomes
Species of your interest not listed? Contact us
Application: Western Blotting
Pudmed ID: 24476911
Journal: Nucleic Acids Res
Figure Number: 6C
Published Date: 2014-04-01
First Author: Gunnelius, L., Hakkila, K., et al.
Impact Factor: 16.476Open Publication
Photosynthetic properties of ?rpoZ. (A) Light-saturated photosynthetic activity of a 1 ml of culture (OD730 = 1) of CS (white bar) and ?rpoZ (black bar) in standard conditions. Each bar represents an average of three independent biological replicates, and the error bars denote SE. (B) Fluorescence at 77 K was measured using 440-nm light that excites Chl. The data were normalized by dividing with the height of the PSI emission peak at 723 nm. (C) Total proteins were isolated, separated with SDS-PAGE and the amounts of PSI reaction center protein PsaB, PSII core protein CP43 and the phycobilisome proteins allophycocyanin (APC) and phycocyanin (PC) were measured by western blotting. The protein contents of PsaB, CP43, ACP and PC were 99 ą 6%, 97 ą 7, 103 ą 7% and 99 ą 4, respectively, in ?rpoZ of that measured in CS.
Allophycocyanin is a protein of the light-harvesting phycobiliprotein family of red algae and cyanobacteria. It is an accessory pigment to chlorophyll A located in the core of the phycobilisome, and its strong spectral overlap with chlorophyll facilitates energy transfer for photosynthesis.
Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.
Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.
Hernandez-Prieto et al. (2011). The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803: Their involvement in chlorophyll biogenesis for Photosystem II. Bioch.Bioph. Acta.
Gantt & Lipschultz (1974). Phycobilisome structure by immuno-electron microscopy. J. Phycology, Vol. 13:3, pages: 185-192. (immunolocalization)
Gantt & Lipschultz (1974). Phycobilisomes of Porphyridium cruentum: Pigment Analysis. Biochem. 13:2960. Gantt E & C Lipschultz (1977). Probing phycobilisome structure by immuno-electron microscopy. J Phycol. 13:18