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APC | allophycocyanin alpha and beta

AS08 277  |  Clonality:Polyclonal  |  Host:Rabbit  |  Reactivity: Red algae, cyanobacteria

APC | allophycocyanin alpha and beta in the group Antibodies Plant/Algal  / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS08 277)
APC | allophycocyanin alpha and beta



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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

native allophycocyanin alpha and beta purified from Porphyridium cruentum phycobilisomes

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 200-1 : 500 (IL), 1 : 1500 - 3000 (WB)
Expected | apparent MW

14-19 kDa

Reactivity

Confirmed reactivity Porphyridium cruentum, Synechocystis PCC 6803
Predicted reactivity Red Algae, Cyanobacteria
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Reactant: Synechocystis

Application: Western Blotting

Pudmed ID: 24476911

Journal: Nucleic Acids Res

Figure Number: 6C

Published Date: 2014-04-01

First Author: Gunnelius, L., Hakkila, K., et al.

Impact Factor: 16.476

Open Publication

Photosynthetic properties of ?rpoZ. (A) Light-saturated photosynthetic activity of a 1 ml of culture (OD730 = 1) of CS (white bar) and ?rpoZ (black bar) in standard conditions. Each bar represents an average of three independent biological replicates, and the error bars denote SE. (B) Fluorescence at 77 K was measured using 440-nm light that excites Chl. The data were normalized by dividing with the height of the PSI emission peak at 723 nm. (C) Total proteins were isolated, separated with SDS-PAGE and the amounts of PSI reaction center protein PsaB, PSII core protein CP43 and the phycobilisome proteins allophycocyanin (APC) and phycocyanin (PC) were measured by western blotting. The protein contents of PsaB, CP43, ACP and PC were 99 ą 6%, 97 ą 7, 103 ą 7% and 99 ą 4, respectively, in ?rpoZ of that measured in CS.

Additional information

Additional information This product can be sold containing proclin if requested

Related products

Related products

AS08 278 | Anti-R-PC | R-phycocyanin, rabbit antibodies

AS08 279 | Anti-b-PE | phycoerythrobilin, rabbit antibodies

AS08 280 | Anti-B-PE | phycoerythrobilin and phycourobilin, rabbit antibodies

Background

Background

Allophycocyanin is a protein of the light-harvesting phycobiliprotein family of red algae and cyanobacteria. It is an accessory pigment to chlorophyll A located in the core of the phycobilisome, and its strong spectral overlap with chlorophyll facilitates energy transfer for photosynthesis.

Product citations

Selected references Ge at al. (2017). Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017 Jul;16(7):1258-1274. doi: 10.1074/mcp.M116.068080.
Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.
Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.
Hernandez-Prieto et al. (2011). The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803: Their involvement in chlorophyll biogenesis for Photosystem II. Bioch.Bioph. Acta.
Gantt & Lipschultz (1974). Phycobilisome structure by immuno-electron microscopy. J. Phycology, Vol. 13:3, pages: 185-192. (immunolocalization)
Gantt & Lipschultz (1974). Phycobilisomes of Porphyridium cruentum: Pigment Analysis. Biochem. 13:2960. Gantt E & C Lipschultz (1977). Probing phycobilisome structure by immuno-electron microscopy. J Phycol. 13:18

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