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Arabinogalactan-3 (clone CCRC-M85)

AS16 3133-1ml  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Acer pseudoplatanus, Arabidopsis thaliana, Solanum lycopersicum

From the laboratory of Michael G. Hahn, PhD, University of Georgia

Arabinogalactan-3 (clone CCRC-M85) in the group Antibodies Plant/Algal / Cell Wall / Dr. Hahn collection, University of Georgia at Agrisera AB (Antibodies for research) (AS16 3133-1ml)
Arabinogalactan-3 (clone CCRC-M85)



DATA SHEET IN PDF

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246 €
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

MeBSA-conjugated sycamore maple (Acer pseudoplatanus) pectic polysaccharide.
Host Mouse
Clonality Monoclonal
Subclass/isotype IgM
Purity Cell culture supernatant.
Format Liquid
Quantity 1 ml
Storage Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Recommended dilution Undiluted or at 1 : 10 (ELISA), (IF), (IHC)

Reactivity

Confirmed reactivity Acer pseudoplatanus, Arabidopsis thaliana, Solanum lycopersicum
Predicted reactivity Arabinogalactans from gum arabic, gum ghatti, and sycamore and tomato pectic polysaccharides
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Exact working dilution needs to be determined by end user

Related products

Background

Background Arabinogalactans are polymers composed of arabinose and galactose monosaccharides. They exist in plants as free glycans or are attached to rhamnogalacturonan I or to protetein backbones. When attached to proteins they form arabinogalactan protein (AGP) which works as an intercellular signaling molecule. AGP also functions as a glue for sealing wounds in plants.  
Arabinogalactans can be used as an additive in food, and as a replacement for starch in food or pharmaceutical products.

This pectic polysaccharide antibody belongs to the arabinogalactan 3 group of antibodies. Pectin consists of polysaccharides found in the primary cell wall of most plants which binds cells together in the middle lamella.

Product citations

Selected references Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
background: Arabinogalactans are polymers composed of arabinose and galactose monosaccharides. They exist in plants as free glycans or are attached to rhamnogalacturonan I or to protetein backbones. When attached to proteins they form arabinogalactan protein (AGP) which works as an intercellular signaling molecule. AGP also functions as a glue for sealing wounds in plants.  
Arabinogalactans can be used as an additive in food, and as a replacement for starch in food or pharmaceutical products.

This pectic polysaccharide antibody belongs to the arabinogalactan 3 group of antibodies. Pectin consists of polysaccharides found in the primary cell wall of most plants which binds cells together in the middle lamella.
All references: Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
Confirmed reactivity: Acer pseudoplatanus, Arabidopsis thaliana, Solanum lycopersicum
predicted reactivity: Arabinogalactans from gum arabic, gum ghatti, and sycamore and tomato pectic polysaccharides
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Clonality: Monoclonal
Format: Liquid
Host: Mouse
immunogen:

MeBSA-conjugated sycamore maple (Acer pseudoplatanus) pectic polysaccharide.
Purity: Cell culture supernatant.
Quantity: 1 ml
recommended dilution: Undiluted or at 1 : 10 (ELISA), (IF), (IHC)
storage: Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class: IgM
tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
additional information: Exact working dilution needs to be determined by end user