Arabinogalactan-protein, AGP (monoclonal, clone LM2)
AS18 4211-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
DATA SHEET IN PDF| Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
Polysaccharide Arabinogalactan-protein (AGP) from Oryza sativa
Host
Rat
Clonality
Monoclonal
Subclass/isotype
IgM
Purity
Cell culture supernatant.
Format
Liquid
Quantity
1 ml
Storage
Store at +4°C (short term) and at -20°C (long term).
Tested applications
ELISA (ELISA), Immuniflourescence (IF)
Recommended dilution
1:10 (ELISA, IF)
Reactivity
Confirmed reactivity
Higher plants, ferns and mosses
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
Contains 0.05% Sodium Azide.
This antibody is made to rice arabinogalactan-proteins (AGPs) and it recognizes a carbohydrate epitope containing B-linked glucuronic acid.
In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50%) by 70 mg/ml 1-O-methyl-B-D-GlcA. The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-O-methyl-B-D-GlcA.
This antibody is made to rice arabinogalactan-proteins (AGPs) and it recognizes a carbohydrate epitope containing B-linked glucuronic acid.
In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50%) by 70 mg/ml 1-O-methyl-B-D-GlcA. The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-O-methyl-B-D-GlcA.
Antibody is recognizing carbohydrate epitope containing Β-linked glucuronic acid.
Background
Background
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
Product citations
Selected references
Stacey et al. (1990). Patterns of expression of the JIM4 arabinogalactan-protein epitope in cell cultures and during somatic embryogenesis in Daucus carota L Planta. 1990 Jan;180(2):285-92.doi: 10.1007/BF00194009.
Knox et al.(1991). Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J. 1991 ov;1(3):317-326.doi: 10.1046/j.1365-313X.1991.t01-9-00999.x.
Knox et al.(1991). Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J. 1991 ov;1(3):317-326.doi: 10.1046/j.1365-313X.1991.t01-9-00999.x.
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