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ARP8 | Actin-related protein 8, (clone 13F10,A4,G6), N-Terminal

AS20 4509-1ml | Clonality: Monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana
ARP8 | Actin-related protein 8, (clone 13F10,A4,G6), N-Terminal in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS20 4509-1ml)
ARP8 | Actin-related protein 8, (clone 13F10,A4,G6), N-Terminal



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Product Information

Immunogen MAP- conjugated peptide of Arabidopsis thaliana ARP8 N-terminal, UniProt: Q9FKT0-1, TAIR: At5g56180
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Cell culture supernatant
Format Liquid
Quantity 1 ml
Storage Store at -80°C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunocytochemistry (ICC), Immunoflourescence (IF), Western blot (WB)
Recommended dilution assay dependent
Expected | apparent MW 52 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica rapa, Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Antibody is recognizing following epitope, amino acids 2-26:  aa 2-ILKKVWG SVWNRSNSGKDLVNHQRA-26
This antibody is recognizing the full-length 52 kDa recombinant ARP8 protein expressed in Escherichia coli as well as endogenous ARP8 of identical molecular weight in Arabidopsis thaliana extracts from different tissues: all vegetative and reproductive organs examined including seedlings, roots and siliques, with higher concentrations of the protein detected in  developing flower buds and flowers within the inflorescence.

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Background

Background Actin-related proteins (ARPs) are found in the nuclei of all eukaryotic cells, but their functions are generally understood only in the context of their presence in various yeast and animal chromatin-modifying complexes. Arabidopsis ARP8 shows 30 and 29% amino acid identity to yeast actin and Arabidopsis ACT2 in the regions of alignment, respectively. Because it is not closely related to yeast or human ARP8 and shows similar weak homology to yeast ARP8 and ARP9, the Arabidopsis ARP8 is considered a plant-specific orphan ARP.

Product citations

Selected references Kandasamy et al. (2008). ACTIN-RELATED PROTEIN8 encodes an F-box protein localized to the nucleolus in Arabidopsis. Plant Cell Physiol. 49(5):858-63. doi: 10.1093/pcp/pcn053.
immunogen: MAP- conjugated peptide of Arabidopsis thaliana ARP8 N-terminal, UniProt: Q9FKT0-1, TAIR: At5g56180
Sub class: IgG1
Host: Mouse
Clonality: Monoclonal
Purity: Cell culture supernatant
Format: Liquid
Quantity: 1 ml
storage: Store at -80°C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunoflourescence (IF), Western blot (WB)
recommended dilution: assay dependent
calculated | apparent molecular mass [kDa]: 52 kDa
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Brassica rapa, Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information (application): Antibody is recognizing following epitope, amino acids 2-26:  aa 2-ILKKVWG SVWNRSNSGKDLVNHQRA-26
This antibody is recognizing the full-length 52 kDa recombinant ARP8 protein expressed in Escherichia coli as well as endogenous ARP8 of identical molecular weight in Arabidopsis thaliana extracts from different tissues: all vegetative and reproductive organs examined including seedlings, roots and siliques, with higher concentrations of the protein detected in  developing flower buds and flowers within the inflorescence.
background: Actin-related proteins (ARPs) are found in the nuclei of all eukaryotic cells, but their functions are generally understood only in the context of their presence in various yeast and animal chromatin-modifying complexes. Arabidopsis ARP8 shows 30 and 29% amino acid identity to yeast actin and Arabidopsis ACT2 in the regions of alignment, respectively. Because it is not closely related to yeast or human ARP8 and shows similar weak homology to yeast ARP8 and ARP9, the Arabidopsis ARP8 is considered a plant-specific orphan ARP.
All references: Kandasamy et al. (2008). ACTIN-RELATED PROTEIN8 encodes an F-box protein localized to the nucleolus in Arabidopsis. Plant Cell Physiol. 49(5):858-63. doi: 10.1093/pcp/pcn053.

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