ASH1 | Histone-lysine N-methyltransferase ASH1

Product no: AS14 2816

AS14 2816  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Drosophila melanogaster

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  • Product Info
  • Immunogen: N-terminal GST-fusion of the peptide containing amino acids 1756-1855 of the Drosophila melanogaster Ash1protein, UniProt: Q9VW15
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunolocalization (IL), Chromatin Immunoprecipitation (ChIP)
    Recommended dilution: 1 : 500 (IL), 10ng/ul (ChIP)
    Expected | apparent MW:

    236 | 300 kDa

  • Reactivity
  • Confirmed reactivity: Drosophila melanogaster
    Predicted reactivity: Drosophila melanogaster
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Background
  • Background: Histone-lysine N-methyltransferase ash1 is a Trithorax group (TrxG) protein that has histone methyltransferase activity. Specifically trimethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation.
  • Product Citations
  • Selected references: Kahn et al. (2016). Interdependence of PRC1 and PRC2 for recruitment to Polycomb Response Elements. Nucleic Acids Res. 2016 Aug 23. pii: gkw701. [Epub ahead of print].
    Lee et al. (2015). Genome-wide activities of Polycomb complexes control pervasive transcription. Genome Res. 2015 Aug;25(8):1170-81. doi: 10.1101/gr.188920.114. Epub 2015 May 18.
  • Protocols


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