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Epitope tag Antibodies


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ASH1 | Histone-lysine N-methyltransferase ASH1

AS14 2816  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Drosophila melanogaster

ASH1 | Histone-lysine N-methyltransferase ASH1 in the group Human/Animal Antibodies / Drosophila melanogaster at Agrisera AB (Antibodies for research) (AS14 2816)

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product information
Background Histone-lysine N-methyltransferase ash1 is a Trithorax group (TrxG) protein that has histone methyltransferase activity. Specifically trimethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation.
Immunogen N-terminal GST-fusion of the peptide containing amino acids 1756-1855 of the Drosophila melanogaster Ash1protein, UniProt: Q9VW15
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Chromatin Immunoprecipitation (ChIP)
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Secondary antibodies
Additional information
application information
Recommended dilution 1 : 500 (IL), 10ng/ul (ChIP)
Expected | apparent MW

236 | 300 kDa
Confirmed reactivity Drosophila melanogaster
Predicted reactivity Drosophila melanogaster
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Kahn et al. (2016). Interdependence of PRC1 and PRC2 for recruitment to Polycomb Response Elements. Nucleic Acids Res. 2016 Aug 23. pii: gkw701. [Epub ahead of print].
Lee et al. (2015). Genome-wide activities of Polycomb complexes control pervasive transcription. Genome Res. 2015 Aug;25(8):1170-81. doi: 10.1101/gr.188920.114. Epub 2015 May 18.

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