ASH1 | Histone-lysine N-methyltransferase ASH1
AS14 2816 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Drosophila melanogaster

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
N-terminal GST-fusion of the peptide containing amino acids 1756-1855 of the Drosophila melanogaster Ash1protein, UniProt: Q9VW15
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunolocalization (IL), Chromatin Immunoprecipitation (ChIP)
Recommended dilution
1 : 500 (IL), 10ng/ul (ChIP)
Expected | apparent MW
236 | 300 kDa
Reactivity
Confirmed reactivity
Drosophila melanogaster
Predicted reactivity
Drosophila melanogaster
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Background
Background
Histone-lysine N-methyltransferase ash1 is a Trithorax group (TrxG) protein that has histone methyltransferase activity. Specifically trimethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation.
Product citations
Selected references
Kahn et al. (2016). Interdependence of PRC1 and PRC2 for recruitment to Polycomb Response Elements. Nucleic Acids Res. 2016 Aug 23. pii: gkw701. [Epub ahead of print].
Lee et al. (2015). Genome-wide activities of Polycomb complexes control pervasive transcription. Genome Res. 2015 Aug;25(8):1170-81. doi: 10.1101/gr.188920.114. Epub 2015 May 18.
Lee et al. (2015). Genome-wide activities of Polycomb complexes control pervasive transcription. Genome Res. 2015 Aug;25(8):1170-81. doi: 10.1101/gr.188920.114. Epub 2015 May 18.
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