IKP

AtCCaP1 | vacuolar calcium-binding protein-related

Product no: AS09 483

AS09 483 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCaP1m UniProt: Q9SXE9, TAIR: At1g62480

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Storage: Store lyophilized/reconstituted at -20C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 8000 (ELISA), 1 : 1000 (WB)
    Expected | apparent MW: 16,6 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Arabidopsis thaliana
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    westrn blot using anti-CCaP1 antibodies

    5 µg (1,3) and 2.5 µg (2,5) of lysate from E.coli cells expressing CCaP1 protein/lane were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-CCaP1 antibodies (AS09 483, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

  • Additional Information
  • Additional information:

    0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

    Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

    Additional information (application):

    Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

    Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

  • Background
  • Background:

    CCaP1 acts as a mediator in response to continuous dark or gibberellic acid. Alternative name: At1g62480/T3P18_4, T3P18.4

  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection



    Method for isolation of plant vacuolar membranes

    method for vacuolar membrane isolation

    Courtesy of Dr. Masayoshi Maeshima, Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences Nagoya University Nagoya, Japan

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AS07 213  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Higher plants including A.comosus, A.thaliana, C.sativus, C. australis R.Br, C.reinhardtii, F. margarita Swingle, H.vulgare, L.esculentum, L.longiflorum, Malus x domestica Borkh. c.v. Fuji, M. truncatula, M.crystallinum, N.tabacum, N.caerulescens, O.sativa, P.hybrida cv. Mitchell, Populus sp., P.vittata, Thellungiella sp., T. aestivum, Z.mays, V. vinifera |  Cellular [compartment marker] of tonoplast membrane

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AS07 260 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae | Cellular [compartment marker] for plasma membrane

Benefits of using this antibody

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