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ATG5 | Autophagy-related protein 5

AS15 3060 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

ATG5 | Autophagy-related protein 5 in the group Antibodies for Plant/Algal  / Protein Modifications / Autophagy-related and Ubiquitin-like Proteins at Agrisera AB (Antibodies for research) (AS15 3060)

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Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen

Recombinant ATG5 of Arabidopsis thaliana, UniProt:Q9FFI2, TAIR:AT5G17290

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)

Reactivity

Confirmed reactivity Recombinant ATG5 of Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

This antibody is so far recognizing recombinant ATG5 from Arabidopsis thaliana and reactivity on endogenous protein needs to be confrimed. It does not react with 6xHis-ATG7.

This product can be sold containing ProClin if requested.

Related products

Related products

AS14 2811 | Anti-ATG8A | Autophagy-related protein 8a, rabbit antibodies

AS12 1854 | Anti-NPR1 | Nonexpresser of PR genes 1 (Arabidopsis thaliana), rabbit antibodies

AS16 4108 | Anti-NPR1 | Nonexpresser of PR genes 1 (other species)

Collection of antibodies to plant proteasome

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background ATG5 (Autophagy-related protein 5) the protein forms a conjugate with ATG12 with an essential role in plant nutrient recycling. It is involved in complete proteolysis of chloroplast stroma proteins in senescent leaves and degradation of damaged peroxisomes.

Product citations

Selected references Young et al. (2019). A facile forward-genetic screen for Arabidopsis autophagy mutants reveals twenty-one loss-of-function mutations disrupting six ATG genes. Autophagy. 2019 Jun;15(6):941-959. doi: 10.1080/15548627.2019.1569915.

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