ATG5 | Autophagy-related protein 5

AS15 3060 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

Recombinant ATG5 of Arabidopsis thaliana, UniProt:Q9FFI2, TAIR:AT5G17290

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Reactivity

Confirmed reactivity Recombinant ATG5 of Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

This antibody is so far recognizing recombinant ATG5 from Arabidopsis thaliana and reactivity on endogenous protein needs to be confrimed. It does not react with 6xHis-ATG7.

This product can be sold containing ProClin if requested.

Background

Background ATG5 (Autophagy-related protein 5) the protein forms a conjugate with ATG12 with an essential role in plant nutrient recycling. It is involved in complete proteolysis of chloroplast stroma proteins in senescent leaves and degradation of damaged peroxisomes.

Product citations

Selected references Young et al. (2019). A facile forward-genetic screen for Arabidopsis autophagy mutants reveals twenty-one loss-of-function mutations disrupting six ATG genes. Autophagy. 2019 Jun;15(6):941-959. doi: 10.1080/15548627.2019.1569915.

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Confirmed reactivity:	Recombinant ATG5 of Arabidopsis thaliana
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Recombinant ATG5 of Arabidopsis thaliana, UniProt:Q9FFI2, TAIR:AT5G17290
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
All references:	Young et al. (2019). A facile forward-genetic screen for Arabidopsis autophagy mutants reveals twenty-one loss-of-function mutations disrupting six ATG genes. Autophagy. 2019 Jun;15(6):941-959. doi: 10.1080/15548627.2019.1569915.
background:	ATG5 (Autophagy-related protein 5) the protein forms a conjugate with ATG12 with an essential role in plant nutrient recycling. It is involved in complete proteolysis of chloroplast stroma proteins in senescent leaves and degradation of damaged peroxisomes.
additional information:	This antibody is so far recognizing recombinant ATG5 from Arabidopsis thaliana and reactivity on endogenous protein needs to be confrimed. It does not react with 6xHis-ATG7. This product can be sold containing ProClin if requested.