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BetaCA1 | Beta carbonic anhydrase 1 (chloroplastic)

AS19 4321 | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

BetaCA1 | Beta carbonic anhydrase 1 (chloroplastic) in the group Antibodies for Plant/Algal  / Photosynthesis  / RUBISCO/Carbon metabolism at Agrisera AB (Antibodies for research) (AS19 4321)

DATA SHEET IN PDF

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Data sheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated synthetic peptide, derived in the part C-terminus of BetaCA1 of Arabidopsis thaliana, UniProt: P27140, TAIR: At3g01500
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 20 000 (WB)
Expected | apparent MW 37.5 | 25.3 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Solanum lycopersicum Q5NE20
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Extraction method – Grind 50 mg of leaf tissue in a sterile microcentrifuge tube using a sterile plastic pestle. Add 132μL of Protein Extraction Buffer (1x TE, 1.2 %SDS, 2.7% sucrose, 7.5 μg mL-1 bromophenol blue) to the ground leaf tissue. Vortex the sample and keep on ice for 15 mins. Centrifuge at 14,000 rpm for five minutes using a benchtop centrifuge. Collect the supernatant and in a new sterile 0.5 ml microcentrifuge tube and discard the pellet.

This antibody does not recognize betaCA2.

Related products

Related products AS05 073 | Anti-CAH3 | Carbonic anhydrase, rabbit antibodies
AS11 1737 | Anti-Beta-CA1, beta-CA2 | carbonic anhydrase | mitochondrial | Chlamydomonas, rabbit antibodies
AS17 4114 | Anti-Gamma CA | Gamma Carbonic anhydrases

Background

Background BetaCA1 (Beta carbonic anhydrase 1) (chloroplastic) - is a zinc metalloenzyme that interconvert CO2 and HCO3 (-).
Alternative names:  ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3, ATBCA1, ATSABP3, BETA CARBONIC ANHYDRASE 1, CA1, CARBONIC ANHYDRASE 1, SABP3, SALICYLIC ACID-BINDING PROTEIN 3.

Product citations

Selected references DiMario et al. (2016). The Cytoplasmic Carbonic Anhydrases βCA2 and βCA4 Are Required for Optimal Plant Growth at Low CO2. Plant Physiol. 2016 May;171(1):280-93. doi: 10.1104/pp.15.01990.

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