BetaCA1 | Beta carbonic anhydrase 1 (chloroplastic)

AS19 4321 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

BetaCA1 | Beta carbonic anhydrase 1 (chloroplastic)

Product Information

Immunogen KLH-conjugated synthetic peptide, derived in the part C-terminus of BetaCA1 of Arabidopsis thaliana, UniProt: P27140, TAIR: At3g01500

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 20 000 (WB)

Expected | apparent MW 37,5 | 25,3 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Solanum lycopersicum Q5NE20
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Extraction method – Grind 50 mg of leaf tissue in a sterile microcentrifuge tube using a sterile plastic pestle. Add 132μL of Protein Extraction Buffer (1x TE, 1.2 %SDS, 2.7% sucrose, 7.5 μg mL-1 bromophenol blue) to the ground leaf tissue. Vortex the sample and keep on ice for 15 mins. Centrifuge at 14,000 rpm for five minutes using a benchtop centrifuge. Collect the supernatant and in a new sterile 0.5 ml microcentrifuge tube and discard the pellet.

This antibody does not recognize betaCA2.

Background

Background BetaCA1 (Beta carbonic anhydrase 1) (chloroplastic) - is a zinc metalloenzyme that interconvert CO₂ and HCO₃ (-).
Alternative names: ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3, ATBCA1, ATSABP3, BETA CARBONIC ANHYDRASE 1, CA1, CARBONIC ANHYDRASE 1, SABP3, SALICYLIC ACID-BINDING PROTEIN 3.

Product citations

Selected references DiMario et al. (2016). The Cytoplasmic Carbonic Anhydrases βCA2 and βCA4 Are Required for Optimal Plant Growth at Low CO2. Plant Physiol. 2016 May;171(1):280-93. doi: 10.1104/pp.15.01990.

All references:	DiMario et al. (2016). The Cytoplasmic Carbonic Anhydrases βCA2 and βCA4 Are Required for Optimal Plant Growth at Low CO2. Plant Physiol. 2016 May;171(1):280-93. doi: 10.1104/pp.15.01990.
additional information (application):	Extraction method – Grind 50 mg of leaf tissue in a sterile microcentrifuge tube using a sterile plastic pestle. Add 132μL of Protein Extraction Buffer (1x TE, 1.2 %SDS, 2.7% sucrose, 7.5 μg mL-1 bromophenol blue) to the ground leaf tissue. Vortex the sample and keep on ice for 15 mins. Centrifuge at 14,000 rpm for five minutes using a benchtop centrifuge. Collect the supernatant and in a new sterile 0.5 ml microcentrifuge tube and discard the pellet. This antibody does not recognize betaCA2.
background:	BetaCA1 (Beta carbonic anhydrase 1) (chloroplastic) - is a zinc metalloenzyme that interconvert CO₂ and HCO₃ (-). Alternative names: ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3, ATBCA1, ATSABP3, BETA CARBONIC ANHYDRASE 1, CA1, CARBONIC ANHYDRASE 1, SABP3, SALICYLIC ACID-BINDING PROTEIN 3.
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Solanum lycopersicum Q5NE20 Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
calculated \| apparent molecular mass [kDa]:	37,5 \| 25,3 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide, derived in the part C-terminus of BetaCA1 of Arabidopsis thaliana, UniProt: P27140, TAIR: At3g01500
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 20 000 (WB)
Reconstitution:	For reconstitution add 50 µl, of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)