Beta-Actin, (animal), mouse monoclonal

Product no: AS20 4381

AS20 4381 | Clonality: Monoclonal | Host: Mouse | Reactivity: Chicken, human, mouse, rabbit, rat

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Delivery:  3-6 business days
  • Product Info
  • Sub class: IgG2b
    Immunogen: Synthetic peptide derived from the N-terminal of the human beta-actin 
    Host: Mouse
    Clonality: Monoclonal
    Purity: IgG2b purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
    Format: Lyophilized
    Quantity: 100 g
    Storage: Store at -20C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4C for several days to weeks
    Tested applications: Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1: 1000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion
    Expected | apparent MW: 42 kDa
  • Reactivity
  • Confirmed reactivity: Chicken, human, mouse, rabbit, rat
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    Western blot using anti-beta actin monoclonal antibodies

    20 µg of tissue lysate from human (1), mouse (2), rat (3), rabbit (4) 
    were extracted and separated on SDS-PAGE and blotted to a membrane. Primary antibodies were used at a dilution of 1: 1000 (1 µg/ml). Reaction was developed by chemiluminescence. 

  • Additional Information
  • Additional information: 10mM PBS, pH 7,2, with 1% BSA and 0,05% sodium azide
    Additional information (application): This antibody recognizes both the native and denatured forms of Beta-Actin from a wide range of species
  • Background
  • Background: Beta-Actin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism.  Actin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.
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