Beta-Actin, (loading control antibody), mouse monoclonal

AS20 4381 | Clonality: Monoclonal | Host: Mouse | Reactivity: Chicken, human, mouse, rabbit, rat

Product Information

Immunogen Synthetic peptide derived from the N-terminal of the human beta-actin

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG2b

Purity IgG2b purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.

Format Lyophilized

Quantity 100 µg

Storage Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks

Tested applications Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1: 1000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion

Expected | apparent MW 42 kDa

Reactivity

Confirmed reactivity Chicken, human, mouse, rabbit, rat

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

20 µg of tissue lysate from human (1), mouse (2), rat (3), rabbit (4)
were extracted and separated on SDS-PAGE and blotted to a membrane. Primary antibodies were used at a dilution of 1: 1000 (1 µg/ml). Reaction was developed by chemiluminescence.

Additional information

Additional information 10mM PBS, pH 7,2, with 1% BSA and 0,05% sodium azide

This antibody recognizes both the native and denatured forms of Beta-Actin from a wide range of species

Background

Background Beta-Actin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Actin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.

Product citations

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calculated \| apparent molecular mass [kDa]:	42 kDa
Clonality:	Monoclonal
Format:	Lyophilized
Host:	Mouse
immunogen:	Synthetic peptide derived from the N-terminal of the human beta-actin
Purity:	IgG2b purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Quantity:	100 µg
recommended dilution:	1: 1000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion
storage:	Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks
Sub class:	IgG2b
tested applications:	Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Confirmed reactivity:	Chicken, human, mouse, rabbit, rat
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	10mM PBS, pH 7,2, with 1% BSA and 0,05% sodium azide
additional information (application):	This antibody recognizes both the native and denatured forms of Beta-Actin from a wide range of species
background:	Beta-Actin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Actin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.
Picture (footer):	Application example 20 µg of tissue lysate from human (1), mouse (2), rat (3), rabbit (4) were extracted and separated on SDS-PAGE and blotted to a membrane. Primary antibodies were used at a dilution of 1: 1000 (1 µg/ml). Reaction was developed by chemiluminescence.