Beta-Actin, (loading control antibody), mouse monoclonal

AS20 4381 | Clonality: Monoclonal | Host: Mouse | Reactivity: Chicken, human, mouse, rabbit, rat

Beta-Actin, (loading control antibody), mouse monoclonal in the group Antibodies Human Cell Biology / Developmental biology at Agrisera AB (Antibodies for research) (AS20 4381)


How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen Synthetic peptide derived from the N-terminal of the human beta-actin 
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG2b
Purity IgG2b purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Format Lyophilized
Quantity 100 ĩg
Storage Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks
Tested applications Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1: 1000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion
Expected | apparent MW 42 kDa


Confirmed reactivity Chicken, human, mouse, rabbit, rat
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Western blot using anti-beta actin monoclonal antibodies

20 µg of tissue lysate from human (1), mouse (2), rat (3), rabbit (4) 
were extracted and separated on SDS-PAGE and blotted to a membrane. Primary antibodies were used at a dilution of 1: 1000 (1 µg/ml). Reaction was developed by chemiluminescence. 

Additional information

Additional information 10mM PBS, pH 7,2, with 1% BSA and 0,05% sodium azide
This antibody recognizes both the native and denatured forms of Beta-Actin from a wide range of species

Related products

Related products AS16 4027 | Anti-beta-Actin, (cytoplasmic 1), rabbit antibodies


Background Beta-Actin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism.  Actin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.

Product citations

Related products: Beta-Actin, (loading control antibody), mouse monoclonal

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS16 4027 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human
326 €
AS16 4022 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human
388 €
AS09 627 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Mouse IgG (H&L...
199 €