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BoNT-A | Botulinum toxin A

AS16 4086   | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Botulinum toxin A

BoNT-A | Botulinum toxin A in the group Antibodies Plant/Algal  / Toxins / Other compounds at Agrisera AB (Antibodies for research) (AS16 4086)



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Product Information

Immunogen Recombinat C-terminal heavy chain of BoNT/A1, residues 876–1296, which was cloned into a pET28a His-tag vector (Novagen) and overexpressed in E.coli. UniProt: https://www.uniprot.org/uniprot/P10845
Host Rabbit
Clonality Polyclonal
Purity Total IgG. Protein G purified in PBS pH 7.4.
Format Lyophilized
Quantity 0,5 mg
Reconstitution For reconstitution add 250 ĩl, of sterile water
Storage Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA)
Recommended dilution To be determined by end user

Reactivity

Confirmed reactivity Botulinum toxin A heavy chain
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Related products

Background

Background Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. BotA is cleaved into two chains: heavy and light.

Alternative name: Bontoxilysin-A

Product citations

immunogen: Recombinat C-terminal heavy chain of BoNT/A1, residues 876–1296, which was cloned into a pET28a His-tag vector (Novagen) and overexpressed in E.coli. UniProt: https://www.uniprot.org/uniprot/P10845
Reconstitution: For reconstitution add 250 ĩl, of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Total IgG. Protein G purified in PBS pH 7.4.
Format: Lyophilized
Quantity: 0,5 mg
storage: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: ELISA (ELISA)
recommended dilution: To be determined by end user
Confirmed reactivity: Botulinum toxin A heavy chain
not reactive in: No confirmed exceptions from predicted reactivity are currently known
background: Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. BotA is cleaved into two chains: heavy and light.

Alternative name: Bontoxilysin-A

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