Bromelain

AS09 552 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ananas comosus

Product Information

Immunogen

native bromelain isolated and purified from Ananas comosus

Host Rabbit

Clonality Polyclonal

Purity Purified IgG in PBS.

Format Lyophilized

Quantity 10 mg

Reconstitution For reconstitution add 1 ml of sterile destilled water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)

Expected | apparent MW

39 kDa

Reactivity

Confirmed reactivity Ananas comosus

Predicted reactivity Ananas comosus

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography

Background

Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.

Product citations

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additional information:	Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
additional information (application):	The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
background:	Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
Confirmed reactivity:	Ananas comosus
predicted reactivity:	Ananas comosus
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
calculated \| apparent molecular mass [kDa]:	39 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	native bromelain isolated and purified from Ananas comosus
Purity:	Purified IgG in PBS.
Quantity:	10 mg
recommended dilution:	1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Reconstitution:	For reconstitution add 1 ml of sterile destilled water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)