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Product Information
Immunogen
native bromelain isolated and purified from Ananas comosus
Host
Rabbit
Clonality
Polyclonal
Purity
Purified IgG in PBS.
Format
Lyophilized
Quantity
10 mg
Reconstitution
For reconstitution add 1 ml of sterile destilled water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW
39 kDa
Reactivity
Confirmed reactivity
Ananas comosus
Predicted reactivity
Ananas comosus
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
Background
Background
Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
Product citations
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