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Bromelain

AS09 552 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ananas comosus

Bromelain  in the group Antibodies for Plant/Algal  / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS09 552)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

native bromelain isolated and purified from Ananas comosus

Host Rabbit
Clonality Polyclonal
Purity Purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 10 mg
Reconstitution For reconstitution add 1 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW

39 kDa

Reactivity

Confirmed reactivity Ananas comosus
Predicted reactivity Ananas comosus
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Related products

Related products

AS09 553 | anti-bromelain (biotinylated)

AS09 554 | anti-bromelain (affinity purified)

AS09 546 | anti-PPI | papain (biotinylated)

AS09 547 | anti-PPI | papain (affinity purified)

Plant protein extraction buffer

Secondary antibodies

Background

Background

Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.

Product citations

Selected references

to be added when available