c-Myc tag (mouse monoclonal) (clone 9E10)
AS21 4620 | Clonality: Monoclonal | Host: Mouse | Reactivity: c-myc tagged proteins

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Product Information
Immunogen
Conjugated synthetic peptide: AEEQKLISEEDLL derived from the C-terminal region of human c-Myc. UniProt: P01106
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Immunoglobulin purified on Protein A.
Format
Liquid at 1 mg/ml.
Quantity
0,1 mg
Storage
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Flow cytometry (Flowcyt), Immunohistochemistry (IHC), paraffin, Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
5-10 µg/ml (IHC) paraffin, 1-5 µg/ml (IP), 1-4 µg/ml (Flowcyt), 0,5-2 µg/ml (WB)
Reactivity
Confirmed reactivity
c-Myc tagged fusion proteins
Application examples
Application examples

Flow cytometry experiment: detection of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfected HEK-293 cells (black) using mouse monoclonal anti-c-Myc (clone 9E10).
Additional information
Additional information
The antibody 9E10 can be used to detect the c-Myc tag
Immunohistochemistry (paraffin sections): positive control tissue: perfused brain sections, liver, spleen.
Immunoprecipitation note: this antibody will not immunoprecipitate native c-Myc protein.
Flow cytometry note: Intracellular or extracellular staining, depends upon particular expression.
Immunoprecipitation note: this antibody will not immunoprecipitate native c-Myc protein.
Flow cytometry note: Intracellular or extracellular staining, depends upon particular expression.
Background
Background
c-Myc is derived from Myc proto-oncogene protein. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. A short peptide was used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any c-Myc tag containing protein.
Product citations
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