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c-Myc tag (mouse monoclonal) (clone 9E10)

AS21 4620 | Clonality: Monoclonal | Host: Mouse | Reactivity: c-myc tagged proteins
c-Myc tag (mouse monoclonal) (clone 9E10) in the group Tag Antibodies / Myc at Agrisera AB (Antibodies for research) (AS21 4620)

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Product Information

Immunogen Conjugated synthetic peptide: AEEQKLISEEDLL derived from the C-terminal region of human c-Myc. UniProt: P01106
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Purified by protein-A affinity chromatography
Format Liquid at 1 mg/ml
Quantity 0.1 mg
Storage Store at 2-8°C. Do not freeze. Do not use after the expiration date stamped on the label. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Flow cytometry (Flowcyt), Immunohistochemistry (IHC), paraffin,  Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 5-10 µg/ml (IHC) paraffin, 1-5 µg/ml (IP), 1-4 µg/ml (Flowcyt), 0,5-2 µg/ml (WB)

Reactivity

Confirmed reactivity c-Myc tagged fusion proteins

Application examples

Application examples detection of transfected LST-1-c-Myc in HEK-293 cells using anti-c-Myc mouse monoclonal antibodies

Flow cytometry experiment: detection of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfected HEK-293 cells (black) using mouse monoclonal anti-c-Myc (clone 9E10).

Additional information

Immunohistochemistry (paraffin sections): positive control tissue: perfused brain sections, liver, spleen.
Immunoprecipitation note: this antibody will not  immunoprecipitate native c-Myc protein.
Flow cytometry note: Intracellular or extracellular staining, depends upon particular expression.

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Background

Background c-Myc is derived from Myc proto-oncogene protein. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. A short peptide was used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any c-Myc tag containing protein.

Product citations

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