c-Myc tag (mouse monoclonal) (clone 9E10)

Product no: AS21 4620
AS21 4620 | Clonality: Monoclonal | Host: Mouse | Reactivity: c-myc tagged proteins
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  • Product Info
  • Immunogen: Conjugated synthetic peptide: AEEQKLISEEDLL derived from the C-terminal region of human c-Myc. UniProt: P01106
    Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: Immunoglobulin purified on Protein A.
    Format: Liquid at 1 mg/ml.
    Quantity: 0,1 mg
    Storage: Store at 2-8C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Flow cytometry (Flowcyt), Immunohistochemistry (IHC), paraffin,  Immunoprecipitation (IP), Western blot (WB)
    Recommended dilution: 5-10 µg/ml (IHC) paraffin, 1-5 µg/ml (IP), 1-4 µg/ml (Flowcyt), 0,5-2 µg/ml (WB)
  • Reactivity
  • Confirmed reactivity: c-Myc tagged fusion proteins
  • Application Examples
  • detection of transfected LST-1-c-Myc in HEK-293 cells using anti-c-Myc mouse monoclonal antibodies

    Flow cytometry experiment: detection of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfected HEK-293 cells (black) using mouse monoclonal anti-c-Myc (clone 9E10).

  • Additional Information
  • Additional information: The antibody 9E10 can be used to detect the c-Myc tag
    Additional information (application): Immunohistochemistry (paraffin sections): positive control tissue: perfused brain sections, liver, spleen.
    Immunoprecipitation note: this antibody will not  immunoprecipitate native c-Myc protein.
    Flow cytometry note: Intracellular or extracellular staining, depends upon particular expression.
  • Background
  • Background: c-Myc is derived from Myc proto-oncogene protein. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. A short peptide was used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any c-Myc tag containing protein.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Antibody Protocols
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