Calcineurin/EF-hand motif
AS13 2666 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Hordeum vulgare
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Product Information
Immunogen
KLH-conjugated synthetic peptide derived from known calcineurin/EF-hand motif sequences including Arabidopsis thaliana CPK21, UniProt: Q9ZSA2, TAIR: AT4G04720 and fpound in other calcium-dependent protein kinases
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2500 (WB)
Expected | apparent MW
20-45 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Hordeum vulgare, Populus sp., Triticum aestivum
Predicted reactivity
Brassica napus, Theobroma cacao
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application information
0,5µg of purified calcineurin with His-tag was separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:25 000 in TBS-T containing2% milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 300 seconds.
Courtesy of Dr. Lucyna Misztal, Institute of Molecular Biology and Biotechnology, Poznań, Poland
0,5µg of purified calcineurin with His-tag was separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:25 000 in TBS-T containing2% milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 300 seconds.
Courtesy of Dr. Lucyna Misztal, Institute of Molecular Biology and Biotechnology, Poznań, Poland
Additional information
Antibody is recognizing recombinant calcineurin expressed in E.coli.
Background
Background
Calcineurine is a calcium-binding protein involved in signal transduction.
Product citations
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