Calcineurin/EF-hand motif

Name: Calcineurin/EF-hand motif
SKU: AS13 2666
Price: 302 EUR
Availability: InStock

AS13 2666 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Hordeum vulgare

Calcineurin/EF-hand motif in the group Antibodies Plant/Algal / Plant Developmental Biology / Plant Signal Transduction at Agrisera AB (Antibodies for research) (AS13 2666)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from known calcineurin/EF-hand motif sequences including Arabidopsis thaliana CPK21, UniProt: Q9ZSA2, TAIR: AT4G04720 and fpound in other calcium-dependent protein kinases

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 2500 (WB)

Expected | apparent MW

20-45 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Populus sp., Triticum aestivum

Predicted reactivity Brassica napus, Theobroma cacao
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application information

0,5µg of purified calcineurin with His-tag was separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:25 000 in TBS-T containing2% milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 300 seconds.

Courtesy of Dr. Lucyna Misztal, Institute of Molecular Biology and Biotechnology, Poznań, Poland

Additional information

Antibody is recognizing recombinant calcineurin expressed in E.coli.

Background

Calcineurine is a calcium-binding protein involved in signal transduction.

Product citations

immunogen:	KLH-conjugated synthetic peptide derived from known calcineurin/EF-hand motif sequences including Arabidopsis thaliana CPK21, UniProt: Q9ZSA2, TAIR: AT4G04720 and fpound in other calcium-dependent protein kinases
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
recommended dilution:	1 : 2500 (WB)
Expected \| apparent MW:	20-45 kDa
Confirmed reactivity:	Arabidopsis thaliana, Hordeum vulgare, Populus sp., Triticum aestivum
predicted reactivity:	Brassica napus, Theobroma cacao Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application information 0,5µg of purified calcineurin with His-tag was separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:25 000 in TBS-T containing2% milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 300 seconds. Courtesy of Dr. Lucyna Misztal, Institute of Molecular Biology and Biotechnology, Poznań, Poland
additional information (application):	Antibody is recognizing recombinant calcineurin expressed in E.coli.
background:	Calcineurine is a calcium-binding protein involved in signal transduction.