IKP

14,3,3C | 14,3,3-like protein C

Product no: AS08 317

AS08 317  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Hordeum vulgare

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  • Product Info
  • Immunogen:

    synthetic peptide specific to the barley C isoform, conjugated to KLH

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 15 000 (ELISA), 1 : 2000 (WB)
    Expected | apparent MW:

    30 kDa

  • Reactivity
  • Confirmed reactivity: Hordeum vulgare
    Predicted reactivity: Horderum vulgare
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

     

     
     
    SDS-PAGE gel and duplicate western blot of 1 - Hv14.3.3A; 2 - Hv14.3.3B and 3 - Hv14.3.3C, showing anti Hv14.3.3C antibody recognition only Hv14.3.3C protein band.
    AS08 317 western.jpg
  • Additional Information
  • Additional information (application): Specific for barley 14,3,3C isoform, when tested against 14,3,3A, B and C isoforms
  • Background
  • Background:

    14.3.3’s are 30KDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3’s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction have been ascribed to the 14.3.3 family. To date, five barley 14.3.3 homologues have been identified and characterised and named 14.3.3A through E.

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