Anti-14,3,3C | 14,3,3-like protein C
AS08 317 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Hordeum vulgare
- Data sheet
- Anti-14,3,3C | 14,3,3-like protein C
- Product Info
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Immunogen: synthetic peptide specific to the barley C isoform, conjugated to KLH
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 100 µl Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1 : 15 000 (ELISA), 1 : 2000 (WB) Expected | apparent MW: 30 kDa
- Reactivity
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Confirmed reactivity: Hordeum vulgare Predicted reactivity: Horderum vulgare Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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application example
SDS-PAGE gel and duplicate western blot of 1 - Hv14.3.3A; 2 - Hv14.3.3B and 3 - Hv14.3.3C, showing anti Hv14.3.3C antibody recognition only Hv14.3.3C protein band. - Additional Information
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Additional information (application): Specific for barley 14,3,3C isoform, when tested against 14,3,3A, B and C isoforms - Background
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Background: 14.3.3’s are 30KDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3’s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction have been ascribed to the 14.3.3 family. To date, five barley 14.3.3 homologues have been identified and characterised and named 14.3.3A through E.
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Accessories
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AS12 2119 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. platanoides, A. thaliana, C.australis R.Br., L. longiflorum. M. crystallinum, N. tabacum, O. sativa, P. kurroa, P. vulgaris, algae N. oloabundans, Z.mays
AS13 2640 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stoloniferacv. ‘Penncross’,Arabidopsis thaliana, Brassica sp., Cannabis sativa L., Cucumis sativus, Cynara cardunculus, Glycine max, Hordeum vulgare, Nicotiana tabacum, Phaseolus vulgaris, Phoenix dactylifera, Picrorhiza kurroa, Setaria italica, Solanum tuberosum, Triticum aestivum, Zea mays