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14.3.3C | 14.3.3-like protein C

AS08 317  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Hordeum vulgare

14.3.3C | 14.3.3-like protein C in the group Plant/Algal Antibodies / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS08 317)

PRODUCT INFORMATION IN PDF


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Product Information

Immunogen

synthetic peptide specific to the barley C isoform, conjugated to KLH

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 15 000 (ELISA), 1 : 2000 (WB)
Expected | apparent MW

30 kDa

Reactivity

Confirmed reactivity Hordeum vulgare
Predicted reactivity Horderum vulgare
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

 

 
 
SDS-PAGE gel and duplicate western blot of 1 - Hv14.3.3A; 2 - Hv14.3.3B and 3 - Hv14.3.3C, showing anti Hv14.3.3C antibody recognition only Hv14.3.3C protein band.
AS08 317 western.jpg

Additional information

Specific for barley 14.3.3C isoform, when tested against 14.3.3A, B and C isoforms.

Related products

Related products AS12 2119 | 14-3-3 GRF | General regulatory element, rabbit antibody to all GRF isoforms with broad species reactivity

matching secondary antibody for chemiluminescence detection

Background

Background

14.3.3’s are 30KDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3’s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction have been ascribed to the 14.3.3 family. To date, five barley 14.3.3 homologues have been identified and characterised and named 14.3.3A through E.

Product citations

Selected references

to be added when available