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Cas9 | CRISPR-associated endonuclease 9 (polyclonal) - DyLight® 650 conjugated (40 µg)
Product no: AS16 3690-DL650
AS16 3690-DL650 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cas9 from Streptococcus thermophilus
464
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- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6
(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum Format: Liquid in PBS pH 7,4. Quantity: 40 µg Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use. Tested applications: Immunofluorescence (IF), Western blot (WB) Recommended dilution: To be determined by end user. Expected | apparent MW: Depends upon a MW of a protein which is a fusion partner, - Reactivity
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Confirmed reactivity: Staphylococcus aureus Predicted reactivity: Staphylococcus pyogenes UniProt: Q99ZW2 - Background
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Background: Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system, CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.
DyLight® 650 has Amax = 652 nm, Emax = 672 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection
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