Cat2 | Catalase-2

Product no: AS21 4531

AS21 4531 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana, Brassica napus, Oryza sativa

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  • Product Info
  • Immunogen:

    Full length, recombinant catalase 2 from Arabidopsis thaliana catalase-2 UniProt: P25819 TAIR; At4g35090

    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl, of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 56,9 | 55 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Brassica napus, Oryza sativa
    Predicted reactivity: Salicornia brachiata
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western Blot using anti-Plant Catalase 2 antibodies

    Sample description 1- 15 µg of Arabidopsis thaliana Col-0 extract 2- 15 µg of Arabidopsis thaliana cat2 extract (replicate 1) 3- 15 µg of Arabidopsis thaliana cat2 extract (replicate 2)
    MW markers: from Thermo Fisher (Product #26619)

    15 µg of total protein from 4-weeks-old Arabidopsis thaliana plants extracted with Tris-HCl 50 mM pH 7.8, 0.1 mM EDTA, 0.2 % (V/V) Triton X-100; prepared in 0.063 M Tris-HCl buffer, pH 6.8, containing 2 % sodium dodecyl sulfate (SDS; w/v), 10 % glycerol (v/v), 0.006 % bromophenol blue (w/v) and 10 mM DTT; and denatured at 95 °C for 5 min. Proteins were separated on 12 % SDS-PAGE and blotted 1 h to PVDF using semi-dry transfer. Blots were blocked with 3 % (w/v) skimmed milk powder diluted in TBS-T O/N at 4 °C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2.000 for 1 h at RT with agitation in TBS-T + 3 % (w/v) of skimmed milk powder. The antibody solution was decanted and the blot was washed 3 times for 10 min in TBS-T + 3 % (w/v) of skimmed milk powder at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, Product No: AS09 602 lot 2012) diluted to 1:10.000 in TBS-T + 3 % (w/v) of skimmed milk powder for 1 h at RT with agitation. The blot was washed as above but with TBS and developed for 5 min with chemiluminescent detection reagent. Exposure time was 20 seconds.

    Courtesy of Dr. Luisa M. Sandalio, CISIC, Spain

  • Background
  • Background: Cat2 (Catalase 2)  is an enzyme which occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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This product can be purchased in 3 different volumes:

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