Cat | Catalase (algal)

Product no: AS15 2991

AS15 2991 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, Scenedesmus sp.

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  • Product Info
  • Immunogen:

    KLH-conjugated peptide chosen from Chlamydomonas reinhardtii catalase sequence, UniProt: A8J537

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2500 (WB)
    Expected | apparent MW:

    57 kDa

  • Reactivity
  • Confirmed reactivity: Chlamydomonas reinhardtii, Scenedesmus sp.
    Predicted reactivity: Coccomyxa subellipsoidea C-169, Nannochloropsis gaditana, Ulva prolifera, Zosteria marina
    Species of your interest not listed? Contact us
  • Application Examples
  • application information

    western blot using anti-algal catalase antibodies

    5 µg of total protein from Chlamydomonas reinhardtii extracted with 2 % SDS / 50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 500 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was typically 30 seconds.

    Courtesy of Dr. Thomas Roach, University of Innsbruck, Austria
  • Background
  • Background:

    Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen.

  • Product Citations
  • Selected references: Ameri et al. (2020). Aluminium triggers oxidative stress and antioxidant response in the microalgae Scenedesmus sp. J Plant Physiol. 2020 Jan 15;246-247:153114. doi: 10.1016/j.jplph.2020.153114.
    Kong et al. (2018) Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas. Plant Cell. 2018 Aug;30(8):1824-1847. doi: 10.1105/tpc.18.00361
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