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Cat | Catalase (algal)

AS15 2991 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, Scenedesmus sp.

Cat | Catalase (algal) in the group Antibodies for Plant/Algal  / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS15 2991)

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Data sheet Product citations Add review

Product Information

Immunogen

KLH-conjugated peptide chosen from Chlamydomonas reinhardtii catalase sequence, UniProt: A8J537

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2500 (WB)
Expected | apparent MW

57 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii, Scenedesmus sp.
Predicted reactivity Coccomyxa subellipsoidea C-169, Nannochloropsis gaditana, Ulva prolifera, Zosteria marina
Species of your interest not listed? Contact us

Application examples

Application examples application information

western blot using anti-algal catalase antibodies

5 µg of total protein from Chlamydomonas reinhardtii extracted with 2 % SDS / 50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 500 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was typically 30 seconds.

Courtesy of Dr. Thomas Roach, University of Innsbruck, Austria

Additional information

Related products

Related products

AS08 374 | Anti-KatG | catalase peroxidase (HPI), cyanobacterial, rabbit antibodies
AS09 501 | Anti-Cat | Catalase (peroxisomal marker), higher plants, rabbit antibodies

collection of antibodies to Chlamydomonas reinhardtii proteins

Plant protein extraction buffer

Secondary antibodies

Background

Background

Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen.

Product citations

Selected references Ameri et al. (2020). Aluminium triggers oxidative stress and antioxidant response in the microalgae Scenedesmus sp. J Plant Physiol. 2020 Jan 15;246-247:153114. doi: 10.1016/j.jplph.2020.153114.
Kong et al. (2018) Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas. Plant Cell. 2018 Aug;30(8):1824-1847. doi: 10.1105/tpc.18.00361

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