APX1 | Ascorbate peroxidase (algal)
AS15 2992 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

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Product Information
Immunogen
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 ĩl
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 9000 (WB)
Expected | apparent MW
35 kDa
Reactivity
Confirmed reactivity
Chlamydomonas reinhardtii
Predicted reactivity
Coccomyxa subellipsoidea C-169,yanidioschyzon merolae, Micromonas pusilla (strain CCMP1545), Nannochloropsis gaditana, Ostreococcus lucimarinus (strain CCE9901), Ulva fasciata, Volvox carteri
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Galdieria sulphuraria
Application examples
Application examples
Application information


25 µg of total protein from Chlamydomonas reinhardtii extracted with 2 % SDS / 50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2,500 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Sigma) diluted to 1:25,000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was typically 30 seconds.
Courtesy of Dr. Thomas Roach, University of Innsbruck, Austria
Additional information
Background
Background
APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water.
Product citations
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