APX1 | Ascorbate peroxidase (algal)

Product no: AS15 2992

AS15 2992 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

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  • Product Info
  • Immunogen:
    KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii APX sequence, UniProt: O49822
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 9000 (WB)
    Expected | apparent MW:

    35 kDa

  • Reactivity
  • Confirmed reactivity: Chlamydomonas reinhardtii
    Predicted reactivity: Coccomyxa subellipsoidea C-169,yanidioschyzon merolae, Micromonas pusilla (strain CCMP1545), Nannochloropsis gaditana, Ostreococcus lucimarinus (strain CCE9901), Ulva fasciata, Volvox carteri
    Species of your interest not listed? Contact us
    Not reactive in: Galdieria sulphuraria
  • Application Examples
  • Application information

    western blot using anti-algal AOX antibodies
    25 µg of total protein from Chlamydomonas reinhardtii extracted with 2 % SDS / 50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2,500 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Sigma) diluted to 1:25,000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was typically 30 seconds.

    Courtesy of Dr. Thomas Roach, University of Innsbruck, Austria
  • Background
  • Background:

    APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water.

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Accessories

AS15 2991 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, Scenedesmus sp.

302 €
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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

201 €
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AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S

This product can be purchased in 3 different volumes:

AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer

AS16 ECL-SN, 100 ml

AS16 ECL-SN-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 26 €

AS15 2882 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

302 €
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