GPXh | Glutathione peroxidase (algal)

Name: GPXh | Glutathione peroxidase (algal)
SKU: AS15 2882
Price: 302 EUR
Availability: InStock

AS15 2882 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

GPXh | Glutathione peroxidase (algal) in the group Antibodies Plant/Algal / Environmental Stress / Plant Oxidative Stress at Agrisera AB (Antibodies for research) (AS15 2882)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from glutathione peroxidase protein sequence from Chlamydomonas reinhardtii, UniProt: O22448

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 5000 (WB)

Expected | apparent MW

18 | 15 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Volvox carteri
Species of your interest not listed? Contact us

Application examples

Application examples application example

10 µg of a total protein from Chlamydomonas reinhardtii extracted with 2 % SDS/50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed for 30 s with chemiluminescent detection reagent according to the manufacturer's instructions. Exposure time was typically 30 seconds.

Courtesy Dr. Thomas Roach, University of Innsbruck, Austria

Additional information

Background

Background Glutathione peroxidase (GPXh) is an enzyme from glutathione peroxidase family.

Product citations

immunogen:	KLH-conjugated synthetic peptide derived from glutathione peroxidase protein sequence from Chlamydomonas reinhardtii, UniProt: O22448
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 5000 (WB)
calculated \| apparent molecular mass [kDa]:	18 \| 15 kDa
Confirmed reactivity:	Chlamydomonas reinhardtii
predicted reactivity:	Volvox carteri Species of your interest not listed? Contact us
Picture (footer):	application example 10 µg of a total protein from Chlamydomonas reinhardtii extracted with 2 % SDS/50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed for 30 s with chemiluminescent detection reagent according to the manufacturer's instructions. Exposure time was typically 30 seconds. Courtesy Dr. Thomas Roach, University of Innsbruck, Austria
background:	Glutathione peroxidase (GPXh) is an enzyme from glutathione peroxidase family.