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GPXh | Glutathione peroxidase (algal)

AS15 2882 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

GPXh | Glutathione peroxidase (algal) in the group Antibodies for Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS15 2882)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from glutathione peroxidase protein sequence from Chlamydomonas reinhardtii, UniProt: O22448

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

18 | 15 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Volvox carteri
Species of your interest not listed? Contact us

Application examples

Application examples application example

western blot using anti-algal GPXh antibodies


10 µg of a total protein from Chlamydomonas reinhardtii extracted with 2 % SDS/50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed for 30 s with chemiluminescent detection reagent according to the manufacturer's instructions. Exposure time was typically 30 seconds.

Courtesy Dr. Thomas Roach, University of Innsbruck, Austria

Additional information

Related products

Background

Background Glutathione peroxidase (GPXh) is an enzyme from glutathione peroxidase family.

Product citations

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