Cat | Catalase (peroxisomal marker)

AS09 501 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, A.madagascariensis, B.oleracea, H.vulgare, M. perniciosa, N.bentamina, N.tabacum, O.sativa, P.tomentosa, S. italica, S.lycopersicum, S.oleracea, T.aestivum, Z.mays, V. vinifera

Product Information

Immunogen

KLH-conjugated peptide chosen from know plant catalase sequences including Arabidopsis thaliana isoforms: catalase-1 (Q96528, At1g20630), catalase-2 (P25819, At4g35090), catalase-3 (Q42547, At1g20620);

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 2 µg (IP), 1 : 1000 (WB)

Expected | apparent MW

57 | 55 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Aponogeton madagascariensis, Brassica oleracea, Hordeum vulgare, Moniliophthora perniciosa, Musa acuminate, Musa paradisiaca L., Nicotiana bentamina, Nicotiana tabacum, Oryza sativa, Paulownia tomentosa, Setaria italica L. P. Beauv, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera

Predicted reactivity

Avicennia marina, Betula pendula, Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Brassica campestris, Citrus sp., Citrus maxima, Citrus clementina, Camellia sinensis, Cucumis sativus, Elaeis guineensis var. tenera, Eucalyptus grandis, Fragaria ananassa, Glycine max, Gossypium mexicanum, Helianthus annus, Litchi chinensis, Hibiscus cannabinus, Saccharum officinarum, Cucurbita maxima, Cucurbita moschata, Morus notabilis, Nicotiana tabacum, Pinus pinea, Populus jackii, Prunus persica, Raphanus sativus, Sesamum indicum, Paenibacillus sp.

Not reactive in

Chlamydomonas reinhardtii

Application examples

Application example

western blot using anti plant catalase antibody

10 µg of total protein from Arabidopsis thaliana Col0 (1), Cat2-(Col0) (2), Ler0 (3), Cat2-(Ler0) (4), Zea mays (5), Oryza sativa (6), Brassica oleracea (7), Nicotiana bentamina (8) were extracted with 60mM Tris pH 6.9, 10mM DTT, 20% glycerol, 1mm PMSF were separated on 12.5% SDS-PAGE and blotted 1h to PVDF. Blot was blocked with 3% skim milk in PBS+0.05% Tween20 for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in the same buffer. The antibody solution was decanted and the blot was rinsed briefly three times, then washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:50 000 in 3% skim milk in PBS+0.05% Tween20 for 1h at RT with agitation. The blot was washed as above and developed for 1 min with Western Lightning Plus-ECL ( PerkinElmer )according to the manufacturers instructions. Exposure time was 5min. in ChemiDoc XRS+ (Biorad ).

Courtesy of Brigitte van de Cotte, Gent University, Belgium

Additional information

This antibody is recognizing all three isoforms of Arabidopsis thaliana catalase.

This antibody contains 0.1 % ProClin.

To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.

To decrease background signal this antibody needs to be incubated in PBS-T NOT TBS-T.

Related products

Plant protein extraction buffer

Secondary antibodies

Background

Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation.

Product citations

Selected references Calero-Muñoz et al. (2019). Cadmium induces ROS-dependent pexophagy in Arabidopsis leaves. Plant Cell Environ. 2019 May 31. doi: 10.1111/pce.13597.
Szymańska et al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.
Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.
Pan et al. (2018). Comparative proteomic investigation of drought responses in foxtail millet. BMC Plant Biol. 2018 Nov 29;18(1):315. doi: 10.1186/s12870-018-1533-9.
Su et al. (2018). The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome derived signaling in plant development. J Integr Plant Biol. 2018 Mar 25. doi: 10.1111/jipb.12649.
Kang et al. (2018). Autophagy-related (ATG) 11, ATG9 and the phosphatidylinositol 3-kinase control ATG2-mediated formation of autophagosomes in Arabidopsis. Plant Cell Rep. 2018 Jan 19. doi: 10.1007/s00299-018-2258-9.
Mares et al. (2017). Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches' broom disease in cacao. BMC Microbiol. 2017 Aug 17;17(1):176. doi: 10.1186/s12866-017-1085-4.
Sultan et al. (2017). The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria. Plant Cell. 2016 Nov;28(11):2805-2829.
Zhang et al. (2017). Global analysis of protein lysine succinylation profiles in common wheat. BMC Genomics. 2017 Apr 20;18(1):309. doi: 10.1186/s12864-017-3698-2. (Triticum aestivum L., immunoprecipitation)
Kneeshaw et al. (2017). Nucleoredoxin guards against oxidative stress by protecting antioxidant enzymes. Proc Natl Acad Sci U S A. 2017 Jul 19. pii: 201703344. doi: 10.1073/pnas.1703344114.
Dauphinee et al. (2017). Remodelling of lace plant leaves: antioxidants and ROS are key regulators of programmed cell death. Planta. 2017 Apr 7. doi: 10.1007/s00425-017-2683-y. (Aponogeton madagascariensis)
Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.
Lee et al. (2016). Superoxide serves as a putative signal molecule for plant cell division: overexpression of CaRLK1 promotes the plant cell cycle via accumulation of O2 − and decrease in H2O2. Physiol Plantarum. doi:10.1111/ppl.12487