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Cat | Catalase (peroxisomal marker)

AS09 501 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, A.madagascariensis, B.oleracea, H.vulgare, L. albus, M. perniciosa, N.bentamina, N.tabacum, O.sativa, P. zeylandica, P.tomentosa, S. italica, S.lycopersicum, S.oleracea, T.aestivum, Z.mays, V. vinifera

Cat | Catalase (peroxisomal marker) in the group Antibodies for Plant/Algal  / Compartment Markers / Peroxisomal marker at Agrisera AB (Antibodies for research) (AS09 501)

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Product Information

Immunogen

KLH-conjugated peptide chosen from know plant catalase sequences including Arabidopsis thaliana isoforms: catalase-1 (Q96528, At1g20630), catalase-2 (P25819, At4g35090), catalase-3 (Q42547, At1g20620);

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1: 500 (IL), 2 µg (IP), 1 : 1000 (WB)
Expected | apparent MW

57 | 55 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Aponogeton madagascariensis, Brassica oleracea, Hordeum vulgare, Lupinus albus, Moniliophthora perniciosa, Musa acuminate, Musa paradisiaca L., Nicotiana bentamina, Nicotiana tabacum, Oryza sativa, Paulownia tomentosa, Plumbago zeylanica, Setaria italica L. P. Beauv, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera
Predicted reactivity
Avicennia marina, Betula pendula, Brachypodium distachyon, Brassica campestris, Brassica napus, Brassica rapa subsp. pekinensis,  Citrus sp., Citrus clementina, Citrus maxima, Camellia sinensis, Cucumis sativus, Cucurbita maxima, Cucurbita moschata, Elaeis guineensis var. tenera, Eucalyptus grandis, Fragaria ananassa, Glycine max, Gossypium mexicanum, Helianthus annus, Hibiscus cannabinus, Litchi chinensis, Lupinus albus, Manihot esculenta, Morus notabilis, Nicotiana tabacum, Paenibacillus sp., Pinus pinea, Populus jackii, Prunus persica, Raphanus sativus, Saccharum officinarum, Sesamum indicum

Species of your interest not listed? Contact us
Not reactive in

Chlamydomonas reinhardtii

Application examples

Application examples

Application example

western blot using anti plant catalase antibody

10 µg of total protein from Arabidopsis thaliana Col0 (1), Cat2-(Col0) (2), Ler0 (3), Cat2-(Ler0) (4), Zea mays (5), Oryza sativa (6), Brassica oleracea (7), Nicotiana bentamina (8) were extracted with 60mM Tris pH 6.9, 10mM DTT, 20% glycerol, 1mm PMSF were separated on 12.5% SDS-PAGE and blotted 1h to PVDF. Blot was blocked with 3% skim milk in PBS+0.05% Tween20 for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in the same buffer. The antibody solution was decanted and the blot was rinsed briefly three times, then washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:50 000 in 3% skim milk in PBS+0.05% Tween20 for 1h at RT with agitation. The blot was washed as above and developed for 1 min with Western Lightning Plus-ECL ( PerkinElmer )according to the manufacturers instructions. Exposure time was  5min. in ChemiDoc XRS+ (Biorad ).

Courtesy of Brigitte van de Cotte, Gent University, Belgium

Western blot with anti-catalase antibodies

Blots were performed from 10 µg of protein from total extracts, crude extracts as well as from isolated tagged-mitochondria (leaves or roots). Arabidopsis thaliana protein extracts were prepared using a protein extraction buffer (100 mM Tris-HCl pH 7.5, 50 mM EDTA, 250 mM NaCl, 0.05% SDS). Samples were denatured with Laemmli buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol at 95°C for 10 min before separating the protein mixtures on reducing 12% polyacrylamide gel. Protein extracts were blotted 1h onto a 0.45 µm nitrocellulose membrane using wet transfer. Blots were blocked with 5% milk for 1h/RT with agitation. Blots were incubated with the primary antibodies anti-catalase (Agrisera, AS09 501) at a dilution of 1: 1 000 for ON/4°C with agitation in TBS-T or PBS-T + 2% milk, respectively. Blots were washed 3 times for 5 min in TBS-T or PBS-T at RT with agitation. Blot was incubated for 1h/RT with agitation in Agrisera matching secondary antibodies (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:10 000 in TBS-T or PBS-T + 2% milk.  Blots were washed 3 times for 5 min in TBS-T or PBS-T following by 3 additional washing steps for 5 min in TBS or PBS. Visualization was carried out using the chemiluminescence kit Agrisera ECLBright; AS16 ECL-N-100 and signals were detected using Azure c600 Western Blot Imaging system (Azure biosystems). Exposure time was 2-5 min.

Courtesy of Dr Jonathan Przybyla-Toscano, Umeå Plant Science Centre, Sweden

Additional information

Additional information
This antibody is recognizing all three isoforms of Arabidopsis thaliana catalase. Catalase-2 is a main isoform expressed in leaf tissue and localized to peroxisomes. 

This antibody contains 0.1 % ProClin.

To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.

To decrease background signal this antibody needs to be incubated in PBS-T NOT TBS-T. For reference, check image in application example below.

Related products

Related products

AS15 2991 | anti-Cat | Catalase (algal), rabbit antibodies

AS08 374 | anti-KatG | catalase peroxidase (HPI), cyanobacterial, rabbit antibodies 

AS09 501PRE | Cat | Catalase (peroxisomal marker), pre-immune serum

Plant protein extraction buffer

Secondary antibodies

Background

Background

Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation.

Product citations

Selected references Tokarz et al. (2020). Can Ceylon Leadwort ( Plumbago zeylanica L.) Acclimate to Lead Toxicity?-Studies of Photosynthetic Apparatus Efficiency. Int J Mol Sci. 2020 Mar 9;21(5):1866.doi: 10.3390/ijms21051866.
Boussardon et al. (2020). Tissue-specific Isolation of Arabidopsis/plant Mitochondria - IMTACT (Isolation of Mitochondria Tagged in Specific Cell Types). Plant J. 2020 Feb 14. doi: 10.1111/tpj.14723. Online ahead of print.
Rodriguez et al. (2020). Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells. bioRxiv doi.org/10.1101/747410
Calero-Muñoz et al. (2019). Cadmium induces reactive oxygen species-dependent pexophagy in Arabidopsis leaves. Plant Cell Environ. 2019 Sep;42(9):2696-2714. doi: 10.1111/pce.13597.
Mares et al. (2020). Hydrosoluble phylloplane components of Theobroma cacao modulate the metabolism of Moniliophthora perniciosa spores during germination.Fungal Biol. 2020 Jan;124(1):73-81. doi: 10.1016/j.funbio.2019.11.008.
Calero-Muñoz et al. (2019). Cadmium induces ROS-dependent pexophagy in Arabidopsis leaves. Plant Cell Environ. 2019 May 31. doi: 10.1111/pce.13597.
Szymańska et al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.
Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.
Pan et al. (2018). Comparative proteomic investigation of drought responses in foxtail millet. BMC Plant Biol. 2018 Nov 29;18(1):315. doi: 10.1186/s12870-018-1533-9.
Su et al. (2018). The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome derived signaling in plant development. J Integr Plant Biol. 2018 Mar 25. doi: 10.1111/jipb.12649.
Kang et al. (2018). Autophagy-related (ATG) 11, ATG9 and the phosphatidylinositol 3-kinase control ATG2-mediated formation of autophagosomes in Arabidopsis. Plant Cell Rep. 2018 Jan 19. doi: 10.1007/s00299-018-2258-9.
Mares et al. (2017). Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches' broom disease in cacao. BMC Microbiol. 2017 Aug 17;17(1):176. doi: 10.1186/s12866-017-1085-4.
Sultan et al. (2017). The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria. Plant Cell. 2016 Nov;28(11):2805-2829.
Zhang et al. (2017). Global analysis of protein lysine succinylation profiles in common wheat. BMC Genomics. 2017 Apr 20;18(1):309. doi: 10.1186/s12864-017-3698-2. (Triticum aestivum L., immunoprecipitation)
Kneeshaw et al. (2017). Nucleoredoxin guards against oxidative stress by protecting antioxidant enzymes. Proc Natl Acad Sci U S A. 2017 Jul 19. pii: 201703344. doi: 10.1073/pnas.1703344114.
Dauphinee et al. (2017). Remodelling of lace plant leaves: antioxidants and ROS are key regulators of programmed cell death. Planta. 2017 Apr 7. doi: 10.1007/s00425-017-2683-y. (Aponogeton madagascariensis)
Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.
Lee et al. (2016). Superoxide serves as a putative signal molecule for plant cell division: overexpression of CaRLK1 promotes the plant cell cycle via accumulation of O2 − and decrease in H2O2. Physiol Plantarum. doi:10.1111/ppl.12487

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