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CBP | CREB-binding protein homolog

AS13 2730  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Drosophila melanogaster

CBP | CREB-binding protein homolog in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS13 2730)

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Product Information

Immunogen

Recombinant CBP part, fused to GST, derived from Drosophila melanogaster protein sequence, UniProt: O01368

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 3 µg (ChIP), 1 : 500 (WB)
Expected | apparent MW

331 kDa

Reactivity

Confirmed reactivity Drosophila melanogaster
Predicted reactivity Drosophila melanogaster
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot using anti-Drosophila CBP antibodies
Total nuclear protein from 2x10^6 Drosophila melanogaster cultured cells was separated on 6% SDS-PAGE and blotted to PVDF using tank transfer. The blot was incubated in the primary antibody at a dilution of 1: 500 in 1% BSA, 0.05% Tween-20, 1x PBS for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice with 1x PBS. Blot was incubated in secondary antibody (anti-rabbit IgG AP-conjugated) diluted to 1:10 000 in 1% BSA, 0.05% Tween-20, 1x PBS for 30 min at RT with agitation. The blot was rinsed twice with 1x PBS and equilibrated in detection buffer (100mM NaCl; 10mM Tris-HCl pH9.5; 10mM MgCl2). The blot was incubated in substrate solution: 165ug/ml of BCIP and 330ug/ml of NBT in detection buffer until the signal reached the desired contrast and then rinsed with water and air dried.

Cortesy of Dr. Tatyana Kahn, Umeå University, Sweden

Additional information

Related products

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Secondary antibodies

Background

Background CBP (CREB-binding protein homolog) is a transcriptional coactivator in multiple signal transduction pathways. It might play a role in variety of cellular processes, including differentiation, cellular proliferation, immune response, homeostasis, tumorigenesis, and erganogenesis.

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