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HDEL | Endoplasmic reticulum retention signal (clone 2E7)

AS10 683  |  Clonality: monoclonal  |  Host: Mouse  |  Reactivity:Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata,  S. cerevisiae,  D. melanogaster

HDEL | Endoplasmic reticulum retention signal (clone 2E7) in the group Antibodies Plant/Algal / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS10 683)
HDEL | Endoplasmic reticulum retention signal (clone 2E7)



DATA SHEET IN PDF

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Product Information

Immunogen

Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip protein
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG2B
Purity Total IgG. Protein G purified in PBS pH 7.4.
Format Liquid
Quantity 100 µg
Storage Store at -20°C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunofluorescence (IF), Immunogold (IG), Western blot (WB)
Recommended dilution 1: 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)
Expected | apparent MW

78 kDa

Reactivity

Confirmed reactivity Barnyard Grass (E. crus-galli), Beta vulgaris, Drosophila melanogaster, Gossypium hirsutum, Hordeum vulgare,  Saccharomyces cerevisiae, Sorghum sp., Vigna radiata
Predicted reactivity Higher plants
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml

Antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7.
For western blot results,  immunofluorescence and immunogold images please referr to Napier et al. 1992.

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Background

Background

HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.

Product citations

Selected references Luo et al.  (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697.
Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.
background:

HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
All references: Luo et al.  (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697.
Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.
calculated | apparent molecular mass [kDa]:

78 kDa

Clonality: Monoclonal
Format: Liquid
Host: Mouse
immunogen:

Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip protein
Purity: Total IgG. Protein G purified in PBS pH 7.4.
Quantity: 100 µg
recommended dilution: 1: 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)
storage: Store at -20°C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class: IgG2B
tested applications: Immunofluorescence (IF), Immunogold (IG), Western blot (WB)
Confirmed reactivity: Barnyard Grass (E. crus-galli), Beta vulgaris, Drosophila melanogaster, Gossypium hirsutum, Hordeum vulgare,  Saccharomyces cerevisiae, Sorghum sp., Vigna radiata
predicted reactivity: Higher plants
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information: Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
additional information (application):

Antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7.
For western blot results,  immunofluorescence and immunogold images please referr to Napier et al. 1992.

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