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HDEL | Endoplasmic reticulum retention signal (clone 2E7)

AS10 683  |  Clonality: monoclonal  |  Host: Mouse  |  Reactivity:Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata,  S. cerevisiaeD. melanogaster

HDEL | Endoplasmic reticulum retention signal (clone 2E7) in the group Antibodies for Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS10 683)

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Product Information

Immunogen

Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip protein

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG2B
Purity Protein G purified
Format Liquid
Quantity 100 µg
Storage Store at -20°C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunofluorescence (IF), Immunogold (IG), Western blot (WB)
Recommended dilution 1: 50-1 : 500 (IF), 1 : 100-1. 1000 (WB)
Expected | apparent MW

78 kDa

Reactivity

Confirmed reactivity Barnyard Grass (E. crus-galli), Beta vulgaris, Drosophila melanogaster, Gossypium hirsutum, Hordeum vulgare,  Saccharomyces cerevisiae, Sorghum sp., Vigna radiata
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml.

Antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7.
For western blot results,  immunofluorescence and immunogold images please referr to Napier et al. 1992.

Related products

Related products

AS10 683-25 | Anti-HDEL | endoplasmic reticulum retention signal mouse antibody, 25 µg

AS13 2665 | Anti-KDEL | endoplasmic reticulum retention signal (50 µg), rabbit antibodies

collection of antibodies for endomembrane proteins

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background

HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.

Product citations

Selected references Luo et al.  (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697.
Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.

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