Anti-HDEL | Endoplasmic reticulum retention signal (clone 2E7)

Product no: AS10 683

AS10 683  |  Clonality: monoclonal  |  Host: Mouse  |  Reactivity:Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata,  S. cerevisiaeD. melanogaster

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  • Product Info
  • Sub class: IgG2B

    Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip protein

    Host: Mouse
    Clonality: Monoclonal
    Purity: Total IgG. Protein G purified in PBS pH 7.4.
    Format: Liquid
    Quantity: 100 µg
    Storage: Store at -20°C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunofluorescence (IF), Immunogold (IG), Western blot (WB)
    Recommended dilution: 1: 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)
    Expected | apparent MW:

    78 kDa

  • Reactivity
  • Confirmed reactivity: Barnyard Grass (E. crus-galli), Beta vulgaris, Drosophila melanogaster, Gossypium hirsutum, Hordeum vulgare, Saccharomyces cerevisiae, Sorghum sp., Vigna radiata
    Predicted reactivity: Higher plants
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
    Additional information (application):

    Antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7.
    For western blot results,  immunofluorescence and immunogold images please referr to Napier et al. 1992.

  • Background
  • Background:

    HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.

  • Product Citations
  • Selected references: Luo et al.  (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697.
    Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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