HDEL | Endoplasmic reticulum retention signal (clone 2E7)

Name: HDEL | Endoplasmic reticulum retention signal (clone 2E7)
Brand: IKP
SKU: AS10 683
Price: 439 EUR
Availability: InStock

AS10 683 | Clonality: monoclonal | Host: Mouse | Reactivity:Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata, S. cerevisiae, D. melanogaster

HDEL | Endoplasmic reticulum retention signal (clone 2E7) in the group Antibodies Plant/Algal / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS10 683)

HDEL | Endoplasmic reticulum retention signal (clone 2E7)

Product Information

Immunogen

Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip protein

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG2B

Purity Total IgG. Protein G purified in PBS pH 7.4.

Format Liquid

Quantity 100 µg

Storage Store at -20°C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunofluorescence (IF), Immunogold (IG), Western blot (WB)

Recommended dilution 1: 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)

Expected | apparent MW

78 kDa

Reactivity

Confirmed reactivity Barnyard Grass (E. crus-galli), Beta vulgaris, Drosophila melanogaster, Gossypium hirsutum, Hordeum vulgare, Saccharomyces cerevisiae, Sorghum sp., Vigna radiata

Predicted reactivity Higher plants

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml

Antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7.
For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992.

Background

HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.

Product citations

Selected references Luo et al. (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697.
Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.

immunogen:	Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip protein
Sub class:	IgG2B
Host:	Mouse
Clonality:	Monoclonal
Purity:	Total IgG. Protein G purified in PBS pH 7.4.
Format:	Liquid
Quantity:	100 µg
storage:	Store at -20°C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunofluorescence (IF), Immunogold (IG), Western blot (WB)
recommended dilution:	1: 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)
Expected \| apparent MW:	78 kDa
Confirmed reactivity:	Barnyard Grass (E. crus-galli), Beta vulgaris, Drosophila melanogaster, Gossypium hirsutum, Hordeum vulgare, Saccharomyces cerevisiae, Sorghum sp., Vigna radiata
predicted reactivity:	Higher plants
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
additional information (application):	Antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7. For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992.
background:	HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
All references:	Luo et al. (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697. Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.