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CERK1 | Chitin elicitor receptor kinase 1

AS16 4037 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

CERK1 | Chitin elicitor receptor kinase 1 in the group Antibodies for Plant/Algal  / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS16 4037)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RPS4 sequence, UniProt: A8R7E6, TAIR: At3g21630

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized 
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution

1: 1000 - 1: 3500 (WB)

Expected | apparent MW 67 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arabidopsis thaliana
Not reactive in

Nicotiana benthamiana, Solanum lycopersicum

Application examples

Application examples Application example

Western blot using anti-CERK1 antibodies


Total protein from 16-day old Arabidopsis thaliana Col-0 (wild-type) and cerk1-2 null mutant (Cao et al., 2014). Total protein was extracted using Buffer H (Heese et al., 2007; LaMontagne et al., 2016) except that 40 nM Calyculin was used. 70 µg of total protein was denatured at 65°C for 5 min, separated on an 8% SDS-PAGE and transferred for 1h using a tank transfer system to nitrocellulose membrane. Blots were blocked with 1x PBS (Fisher Scientific BP665-1) + 0.1 %Tween 20 (PBS-T) + 5% milk for 1.5h at room temperature (RT) with agitation. Blots were incubated with primary antibody at a dilution of 1: 1000 overnight at 4°C with agitation in 1x PBS-T + 5% milk. The antibody solution was decanted, and the blot washed four times (1x 5min and 3x 6min) in 1x PBS-T at RT with agitation. The blot was then incubated with secondary antibody (Goat anti Rabbit IgG (H&L)–HRP from Agrisera (AS09 602) diluted to 1:10,000 in 1x PBS-T + 5% milk for 4h at RT with agitation. The blot was washed as above and developed for 5 min with Amersham ECL (RPN2106). Exposure time was about 10 min.

Courtesy of Grant Mc Gowan, Gayani Ekanayke & Dr. Antje Heese, Division of Biochemistry, Interdisciplinary Plant Group (IPG), University of Missouri; Columbia, MO, USA.

References:
  • Cao, Y. et al. (2014). The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1. eLife 2014;3:e03766.
  • Heese, A. et al. (2007). The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants. PNAS. Vol. 104 no. 29 p. 12217–12222.
  • LaMontagne et al., (2016). Isolation of Microsomal Membrane Proteins from Arabidopsis thaliana. Current Protocols in Plant Biology 1:1-18. doi: 10.1002/cppb.20020

Additional information

Additional information This product can be sold containing proclin if requested

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Background

Background CERK1 (Chitin elicitor receptor kinase 1) is a Lysin motif (LysM) receptor kinase that functions as a cell surface receptor in chitin elicitor (chitooligosaccharides) signaling leading to innate immunity toward both biotic and abiotic stresses (e.g. tolerance to salinity, heavy-metal stresses, and Botrytis cinerea infection). Recognizes microbe-derived N-acetylglucosamine (NAG)-containing ligands. Involved in the resistance to pathogenic fungi.

Product citations

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