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CesA4 (IRX5) | Cellulose synthase A catalytic subunit 4 [UDP-forming]

AS12 2582 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Solanum lycopersicum, Solanum tuberosum

CesA4 (IRX5) | Cellulose synthase A catalytic subunit 4 [UDP-forming] in the group Antibodies for Plant/Algal  / Compartment Markers / Cell wall marker at Agrisera AB (Antibodies for research) (AS12 2582)

DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen
Recombinant Arabidopsis thaliana IRX5 fragment, UniProt: Q84JA6,TAIR: At5g44030
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

119.5 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Solanum lycopersicum, Solanum tuberosum
Predicted reactivity Betula luminifera, Brassica napus, Capsella rubella, Eutrema salsugineum, Gossypium hirsutum, Nelumbo nucifera, Noccaea caerulescens, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in

Populus tremula

Application examples

Application examples Application example
 western blot using anti-CesA4 (IRX5) antibodies  500 mg of Col-0 WT Arabidopsis thaliana stem powder extracted by boiling in 2 mL of 3% SDS loading buffer + 100 mM DTT at 95C for 10 min. Extract was spun at max speed to remove debris and supernatant was taken as crude extract. 25 µL of this was loaded on a 4-15% gel run for 50 min, 150v. . Blots were blocked with 5 % milk  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:5000 in  for 2h at RT with agitation. The blot was washed as above and developed for 5 min with high sensitivity chemiluminescent detection reagent according to the manufacturer's instructions. Exposure time was 10 seconds.

Courtesy of Dr. Manoj Kumar, University of Manchester, UK

 


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Additional information

Additional information

This antibody is detecting both, recombinant and edogenous CesA4 (IRX5) protein.

Related products

Related products

AS12 2581 | Anti-CesA7 (IRX3) | Cellulose synthase A catalytic subunit 7 [UDP-forming], rabbit antibodies

AS12 2580 | Anti-CesA8 (IRX1) | Cellulose synthase A catalytic subunit 8 [UDP-forming], rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

CesA4 (IRX5) (EC=2.4.1.12) is a catalytic subunit of xylem-specific cellulose synthase enzyme, involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes. It interacts with CESA7 and CESA8 which is required for a functyional complex and localization in secondary cell wall deposition sites. Expressed in young plants, stems and flowers but not in leaves, roots and shoots. Amounts are increasing as stems mature.

Synonymes: Protein IRREGULAR XYLEM 5, IRX5, NWS2.

Product citations

Selected references Otulak-Kozieł et al. (2018). Plant Cell Wall Dynamics in Compatible and Incompatible Potato Response to Infection Caused by Potato Virus Y (PVYNTN). Int J Mol Sci. 2018 Mar 15;19(3). pii: E862. doi: 10.3390/ijms19030862.
Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323.

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