Name:
Phone:
E-mail:
Address:


 


CesA8 (IRX1) | Cellulose synthase A catalytic subunit 8 [UDP-forming]

AS12 2580 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Solanum lycopersicum

CesA8 (IRX1)  | Cellulose synthase A catalytic subunit 8 [UDP-forming] in the group Antibodies for Plant/Algal  / Compartment Markers / Cell wall marker at Agrisera AB (Antibodies for research) (AS12 2580)

DATA SHEET IN PDF

Qty: 
292
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

recombinant Arabidopsis thaliana IRX1 fragment,UniProt: Q8LPK5,TAIR: At4g18780

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunofluorescence (IF), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

111.5 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Cannabis sativa, Solanum lycopersicum
Predicted reactivity Brassica napus
Species of your interest not listed? Contact us
Not reactive in

Populus sp.

Application examples

Application examples application example

 western blot using anti-CesA(IRX1) antibodies 500 mg of Col-0 WT Arabidopsis thaliana stem powder extracted by boiling in 2 mL of 3% SDS loading buffer + 100 mM DTT at 95C for 10 min. Extract was spun at max speed to remove debris and supernatant was taken as crude extract. 25 µL of this was loaded on a 4-15% gel run for 50 min, 150v. . Blots were blocked with 5 % milk  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:5000 in  for 2h at RT with agitation. The blot was washed as above and developed for 5 min with high sensitivity chemiluminescent detection reagent according to the manufacturer's instructions. Exposure time was10 seconds.

Courtesy of Dr. Manoj Kumar, University of Manchester, UK


 

Additional information

Additional information This antibody is detecting both, recombinant and edogenous CesA8 (IRX1) protein.

Related products

Related products

AS12 2582 | Anti-CesA4 (IRX5) | Cellulose synthase A catalytic subunit 4 [UDP-forming], rabbit antibodies

AS12 2581 | Anti-CesA7 (IRX3) | Cellulose synthase A catalytic subunit 7 [UDP-forming], rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

CesA8 (IRX1) is a catalytic subunit of an enzyme involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes. It interacts with CESA4 and CESA7 which is required for a functyional complex. The protein is needed for xylem cell wall thickening. Occurs in secondary cell wall developing tissues such as xylems and interfascicular regions. Not expressed in leaves and not found in embryos.

Synonymes: Protein IRREGULAR XYLEM 1, IRX1, Protein LEAF WILTING 2, LEW2 .

Product citations

Related products: CesA8 (IRX1) | Cellulose synthase A catalytic subunit 8 [UDP-forming]

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS12 2582 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Solanum lyc...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS12 2581 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Solanum lyc...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

Off...
194 €