CesA8 (IRX1) | Cellulose synthase A catalytic subunit 8 [UDP-forming]

Product no: AS12 2580

AS12 2580 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Solanum lycopersicum

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  • Product Info
  • Immunogen:

    recombinant Arabidopsis thaliana IRX1 fragment,UniProt: Q8LPK5,TAIR: At4g18780

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 111,5 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Cannabis sativa, Solanum lycopersicum
    Predicted reactivity: Brassica napus, Lupinus luteus
    Species of your interest not listed? Contact us
    Not reactive in:

    Populus sp.

  • Application Examples
  • application example

     western blot using anti-CesA(IRX1) antibodies 500 mg of Col-0 WT Arabidopsis thaliana stem powder extracted by boiling in 2 mL of 3% SDS loading buffer + 100 mM DTT at 95C for 10 min. Extract was spun at max speed to remove debris and supernatant was taken as crude extract. 25 µL of this was loaded on a 4-15% gel run for 50 min, 150v. . Blots were blocked with 5 % milk  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:5000 in  for 2h at RT with agitation. The blot was washed as above and developed for 5 min with high sensitivity chemiluminescent detection reagent according to the manufacturer's instructions. Exposure time was10 seconds.

    Courtesy of Dr. Manoj Kumar, University of Manchester, UK


     

    Application examples:

    Reactant: Solanum lycopersicum (Tomato)

    Application: Immunocytochemistry-immunoflourescence

    Pudmed ID: 26029225

    Journal: Front Plant Sci

    Figure Number: 7A

    Published Date: 2015-06-02

    First Author: Tsuchiya, M., Satoh, S., et al.

    Impact Factor: 5.435

    Open Publication

    Immunolocalization of CesA4, CesA7 and CesA8 epitopes in the AZs of mature green (MG) and over-ripe (OR) fruit pedicels. Longitudinal sections of MG fruit pedicels, including the AZ, were stained with the anti-CesA4, 7, and 8 antibodies. Micrographs show the negative control without the first antibody step. Phloroglucinol staining for lignin of AZs in MG and OR fruit pedicels. Arrowheads indicate the AZs. Bar = 0.5 mm.

  • Additional Information
  • Additional information: This antibody is detecting both, recombinant and edogenous CesA8 (IRX1) protein
  • Background
  • Background:

    CesA8 (IRX1) is a catalytic subunit of an enzyme involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes. It interacts with CESA4 and CESA7 which is required for a functyional complex. The protein is needed for xylem cell wall thickening. Occurs in secondary cell wall developing tissues such as xylems and interfascicular regions. Not expressed in leaves and not found in embryos.

    Synonymes: Protein IRREGULAR XYLEM 1, IRX1, Protein LEAF WILTING 2, LEW2 .

  • Product Citations
  • Selected references: Zhang et al. (2016). Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis. Nat Commun. 2016 Jun 9;7:11656. doi: 10.1038/ncomms11656.
    Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323.
  • Protocols


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AS12 2581 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Solanum lycopersicum

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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
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