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Anti-CesA8 (IRX1), Cellulose synthase A catalytic subunit 8 [UDP-forming]
AS12 2580 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Solanum lycopersicum
- Product Info
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Immunogen: Recombinant Arabidopsis thaliana IRX1 fragment, UniProt: Q8LPK5,TAIR: At4g18780
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunofluorescence (IF), Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 111.5 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Cannabis sativa, Solanum lycopersicum Predicted reactivity: Brassica napus, Lupinus luteus, Oryza sativa
Species of your interest not listed? Contact usNot reactive in: Populus sp.
- Application Examples
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Application example
500 mg of Col-0 WT Arabidopsis thaliana stem powder extracted by boiling in 2 mL of 3% SDS loading buffer + 100 mM DTT at 95C for 10 min. Extract was spun at max speed to remove debris and supernatant was taken as crude extract. 25 µL of this was loaded on a 4-15% gel run for 50 min, 150v. . Blots were blocked with 5 % milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:5000 in for 2h at RT with agitation. The blot was washed as above and developed for 5 min with high sensitivity chemiluminescent detection reagent according to the manufacturer's instructions. Exposure time was10 seconds.
Courtesy of Dr. Manoj Kumar, University of Manchester, UK
Application examples: 
Reactant: Solanum lycopersicum (Tomato)
Application: Immunocytochemistry-immunoflourescence
Pudmed ID: 26029225
Journal: Front Plant Sci
Figure Number: 7A
Published Date: 2015-06-02
First Author: Tsuchiya, M., Satoh, S., et al.
Impact Factor: 5.435
Open PublicationImmunolocalization of CesA4, CesA7 and CesA8 epitopes in the AZs of mature green (MG) and over-ripe (OR) fruit pedicels. Longitudinal sections of MG fruit pedicels, including the AZ, were stained with the anti-CesA4, 7, and 8 antibodies. Micrographs show the negative control without the first antibody step. Phloroglucinol staining for lignin of AZs in MG and OR fruit pedicels. Arrowheads indicate the AZs. Bar = 0.5 mm.
- Additional Information
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Additional information: This antibody is detecting both, recombinant and edogenous CesA8 (IRX1) protein - Background
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Background: CesA8 (IRX1) is a catalytic subunit of an enzyme involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes. It interacts with CESA4 and CESA7 which is required for a functyional complex. The protein is needed for xylem cell wall thickening. Occurs in secondary cell wall developing tissues such as xylems and interfascicular regions. Not expressed in leaves and not found in embryos.
Synonymes: Protein IRREGULAR XYLEM 1, IRX1, Protein LEAF WILTING 2, LEW2 . - Product Citations
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Selected references: Zhang et al. (2016). Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis. Nat Commun. 2016 Jun 9;7:11656. doi: 10.1038/ncomms11656.
Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323. - Protocols
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS12 2581 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Solanum lycopersicum
AS12 2582 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Solanum lycopersicum, Solanum tuberosum