Chicken anti-Goat IgG (H&L), DyLight® 800 conjugated, min, cross-reactivity to human, mouse or rabbit IgG and serum proteins

Product no: AS12 2376

AS12 2376 | Clonality: Polyclonal | Host: Chicken | Reactivity: Goat IgG (H&L)

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  • Product Info
  • Immunogen:

    Purified Goat IgG, whole molecule

    Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified chicken IgY.
    Format: Lyophilized
    Quantity: 0,5 mg
    Reconstitution: For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    Recommended dilution: 1 : 20-1 : 2000 for most applications
  • Reactivity
  • Not reactive in:
  • Application Examples
  • Additional Information
  • Additional information:

    Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

    Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG, light chains on all goat immunoglobulins

    No reactivity is observed to: non-immunoglobulin goat serum proteins, IgG/serum from human, mouse or rabbit.

    BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

    Additional information (application):
  • Background
  • Background:

    Chicken anti-Goat IgG (H&L), DyLight®800 Conjugated, min. cross-reactivity to human, mouse or rabbit IgG and serum proteins is a secondary antibody conjugated to DyLight® 800, which binds to Goat IgG (H&L) in immunological assays.

    DyLight® 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Goat IgG (H&L)

    DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Antibody protocols
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