Chicken anti-Goat IgG (H&L), DyLight® 800 conjugated, min, cross-reactivity to human, mouse or rabbit IgG and serum proteins

Name: Chicken anti-Goat IgG (H&L), DyLight® 800 conjugated, min, cross-reactivity to human, mouse or rabbit IgG and serum proteins
SKU: AS12 2376
Price: 220 EUR
Availability: InStock

AS12 2376 | Clonality: Polyclonal | Host: Chicken | Reactivity: Goat IgG (H&L)

Chicken anti-Goat IgG (H&L), DyLight® 800 conjugated, min, cross-reactivity to human, mouse or rabbit IgG and serum proteins in the group Secondary Antibodies / Anti-Goat / Fluorescent / DyLight® / DyLight® 800 at Agrisera AB (Antibodies for research) (AS12 2376)

Product Information

Immunogen

Purified Goat IgG, whole molecule

Host Chicken

Clonality Polyclonal

Purity Immunogen affinity purified chicken IgY.

Format Lyophilized

Quantity 0,5 mg

Reconstitution For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Not reactive in

Application examples

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG, light chains on all goat immunoglobulins

No reactivity is observed to: non-immunoglobulin goat serum proteins, IgG/serum from human, mouse or rabbit.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Chicken anti-Goat IgG (H&L), DyLight®800 Conjugated, min. cross-reactivity to human, mouse or rabbit IgG and serum proteins is a secondary antibody conjugated to DyLight® 800, which binds to Goat IgG (H&L) in immunological assays.

DyLight® 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Goat IgG (H&L)

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

immunogen:	Purified Goat IgG, whole molecule
Reconstitution:	For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Host:	Chicken
Clonality:	Polyclonal
Purity:	Immunogen affinity purified chicken IgY.
Format:	Lyophilized
Quantity:	0,5 mg
storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
recommended dilution:	1 : 20-1 : 2000 for most applications
not reactive in:
Picture (footer):
additional information:	Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG, light chains on all goat immunoglobulins No reactivity is observed to: non-immunoglobulin goat serum proteins, IgG/serum from human, mouse or rabbit. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
additional information (application):
background:	Chicken anti-Goat IgG (H&L), DyLight®800 Conjugated, min. cross-reactivity to human, mouse or rabbit IgG and serum proteins is a secondary antibody conjugated to DyLight® 800, which binds to Goat IgG (H&L) in immunological assays. DyLight® 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Goat IgG (H&L) DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

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