Chicken anti-Rabbit IgG (H&L), ALP conjugated
AS10 829 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (heavy and light chains - H&L)
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Product Information
Immunogen
Purified Rabbit IgG
Host
Chicken
Clonality
Polyclonal
Purity
Affinity purified chicken IgY
Format
Liquid
Quantity
1 mg
Storage
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Reactivity
Confirmed reactivity
Rabbit IgG (H&L)
Predicted reactivity
Rabbit IgG (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
This antibody reacts with alpha (heavy chains) on rabbit IgG and all light chains on rabbit immunoglobulins based on immunoelectrophoresis.
No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
Background
Background
Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.
Product citations
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