Chicken anti-Rabbit IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse IgG

AS10 839 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (H&L)


product information
Background		Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Immunogen		Purified Rabbit IgG
Host		Chicken
Clonality		Polyclonal
Clone
Purity		Affinity purified chicken IgY
Format		Lyophilized
Quantity		0.5 mg
Reconstitution		For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications		ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Related products		other antibodies to rabbit IgG HRP conjugated
Additional information		HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

application information
Recommended dilution		The optimal working dilution should be determined by the investigator.
Expected \| apparent MW
Confirmed reactivity		Rabbit IgG (H&L)
Predicted reactivity		Rabbit IgG (H&L)
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse serum and IgG on immunoelectrophoresis. This antibody was absorbed against solid phase human and mouse serum and IgG.
Selected references		to be added when available

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