Chicken anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to human, mouse IgG

Product no: AS10 839

AS10 839 | Clonality: Polyclonal  |  Host: Chicken  | Reactivity: Rabbit IgG (H&L)

218
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    Purified Rabbit IgG

    Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified chicken IgY.
    Format: Lyophilized
    Quantity: 0,5 mg
    Reconstitution: For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: The optimal working dilution should be determined by the investigator
  • Reactivity
  • Confirmed reactivity: Rabbit IgG (H&L)
    Predicted reactivity: Rabbit IgG (H&L)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

    0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

    Additional information (application):

    This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

    No reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse serum and IgG on immunoelectrophoresis.

    This antibody was absorbed against solid phase human and mouse serum and IgG.

  • Background
  • Background:

    Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.

  • Product Citations
  • Selected references: Vergara-Cruces (2024). Structure of the plant plastid-encoded RNA polymerase. Cell . 2024 Feb 29;187(5):1145-1159.e21. doi: 10.1016/j.cell.2024.01.036.
  • Protocols
  • Antibody protocols
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS10 1267 | Clonality: Polyclonal  |  Host:Chicken   |  Reactivity: Rabbit IgG (H&L)

246 €
Add to cart
Info

AS10 829 | Clonality: Polyclonal  |  Host: Chicken  | Reactivity: Rabbit IgG (heavy and light chains - H&L)

281 €
Add to cart
Info

AS10 831 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (H&L)

260 €
Add to cart
Info

AS12 2072 | Clonality: Polyclonal  |  Host: Chicken   |   Reactivity: Rabbit IgG (H&L)

276 €
Add to cart
Info
Close