Chicken anti-Rat IgG (H&L), HRP conjugated, min. cross-reactivity to human, rabbit IgG/serum

AS10 1107 | Clonality: Polyclonal | Host:Chicken | Reactivity:Rat IgG (H&L)


product information
Background		Chicken anti-rat IgG (H&L),HRP conjugated, is a secondary antibody which binds to all rat IgGs in immunological assays. Antibodies are adsorbed against to human,rabbit IgG/serum and affinity purified using solid phase rat IgG.
Host		Chicken
Clonality		Polyclonal
Clone
Purity		Affinity purified IgY
Format		Lyophilized
Quantity		0.5 mg
Reconstitution		For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products		other HRP conjugated secondary antibodies to rat IgG
Additional information		This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins and human and rabbit IgG/serm based on immunoelectrophoresis. Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.

application information
Recommended dilution		1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
Expected \| apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com