Chicken anti-Rat IgG (H&L), HRP conjugated, min. cross-reactivity to human, rabbit IgG/serum
AS10 1107 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rat IgG (H&L)
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Product Information
Host
Chicken
Clonality
Polyclonal
Purity
Affinity purified IgY
Format
Lyophilized
Quantity
0.5 mg
Reconstitution
For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage
Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution
1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
Reactivity
Application examples
Additional information
Additional information
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins and human and rabbit IgG/serm based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Background
Background
Chicken anti-rat IgG (H&L),HRP conjugated, is a secondary antibody which binds to all rat IgGs in immunological assays. Antibodies are adsorbed against to human,rabbit IgG/serum and affinity purified using solid phase rat IgG.
Product citations
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