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Chicken anti-Rat IgG (H&L), HRP conjugated, min, cross-reactivity to human, rabbit IgG/serum
AS10 1107 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rat IgG (H&L)
10 % until end of 2023. Use discount code: Conj10
- Product Info
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Host: Chicken Clonality: Polyclonal Purity: Immunogen affinity purified IgY. Format: Lyophilized Quantity: 0,5 mg Reconstitution: For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Recommended dilution: 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB) - Additional Information
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Additional information: This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins and human and rabbit IgG/serm based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
- Background
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Background: Chicken anti-rat IgG (H&L),HRP conjugated, is a secondary antibody which binds to all rat IgGs in immunological assays. Antibodies are adsorbed against to human,rabbit IgG/serum and affinity purified using solid phase rat IgG. - Protocols
- Antibody protocols
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