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ClpB-P | ClpB3

AS09 459 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Arabidopsis thaliana

ClpB-P | ClpB3 in the group Antibodies for Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS09 459)

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Product Information

Immunogen

KLH-conjugated peptide derived from ClpB-P of Arabidopsis thaliana Q9LF37, At5g15450

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

108.9 | 100 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Populus trichocarpa, Ricinus communis, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot detection using anti-ClpB-P                antibodies

10 µg of total protein from 7 day old Arabidopsis plants grown at control conditions, 22°C wilde type, Columbia-9 (1), hot1-3, HSP101 null (2), clpBp-1, ClpBp null (3) and heat shocked at 38°C for 1.5 hour wilde type, Columbia-9 (4), hot1-3, HSP101 null (5), clpBp-1, ClpBp null (6) were separated on 7-16 % SDS-PAGE and blotted overnight to nitrocellulose membrane. Blots were blocked immediately following transfer in for 1h at room temperature with agitation. Blots were incubated in Agrisera anti-ClpB-P antibody (AS09 459) at a dilution of 1: 1000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:2500 in 5% dry milk blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Exposure time was 20 seconds.

Courtesy Minsoo Kim, University of Arizona, USA

 

Additional information

Additional information There is a cross-reacting band at ca. 95 kDa - therefore longer electrophoresis time is adviced to obtain good separation.

Related products

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AS15 3077 | Anti-pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1, rabbit antibodies
AS07 253 | Anti-HSP101 | ClpB heat shock protein, N-terminal, rabbit antibodies
AS08 287 | Anti-HSP101 | ClpB heat shock protein, C-terminal, rabbit antibodies
AS08 355 | Anti-slr0156 | ATP-dependent chaperone clpB, rabbit antibodies
AS08 344 | Anti-slr1641 | ATP-dependent chaperone clpB, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

ClpB-P is involved in chloroplast biogenesis. Synonymes: ALBINO AND PALE GREEN 6, APG6, AT5G15450, CASEIN LYTIC PROTEINASE B3, CASEIN LYTIC PROTEINASE B-P, CLPB3, CLPB-P, T20K14_60, T20K14.60

Product citations

Selected references Han et al. (2015). A nuclear-encoded chloroplast-targeted S1 RNA-binding domain protein affects chloroplast rRNA processing and is crucial for the normal growth of Arabidopsis thaliana. Plant J. 2015 Jul;83(2):277-89. doi: 10.1111/tpj.12889. Epub 2015 Jun 15.

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