CNfu1 | NifU-like protein 1 (chloroplastic)
AS13 2642 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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3 μg of total protein from Arabidopsis thaliana extracted with were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 10% skimmed milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed twice for 15 min and 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (Goat anti Rabbit HRP) diluted to 1:15 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.
Primary antibody can be also incubated with a good result for 1h/RT.
Courtesy of Dr. Rikard Fristedt, UCLA, USA
Alternative names: CNfu1, AnfU-IVb.
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