CO | Constans (chicken antibody)

AS16 3958 | Clonality: Polyclonal | Host: Chicken | Reactivity: Arabidopsis thaliana

Product Information

Immunogen KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CO protein, UniProt: Q39057, TAIR: At5g15840

Host Chicken

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution for reconstitution please add 50 µl of dest, water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1: 1000 (WB)

Expected | apparent MW 41,9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana (recombinant CO)

Predicted reactivity Camelina sativa, Chrysanthemum sp., Solanum lycopersicum

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Recommended protocol for CO protein extraction Serrano-Bueno et al. 2020. Detection has to be done on a nuclear extract preparation in the evening on a Long Day that is the moment when CO protein is more stable.

Background

Background CO (CONSTANS) is a putative transcription factor which is involved in the long day flowering pathway and has been implied to control mediation between circadian clock and flowering. It regulates flowering time by interactions with SOC1 (suppressor of CO 1), TF1 (terminal flower 1), and FT (flowering locus T). Furthermore it is involved in proline and ethylene biosynthesis.

Product citations

Selected references To be added when available, antibody released in February 2021.

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All references:	To be added when available, antibody released in February 2021.
additional information:	Recommended protocol for CO protein extraction Serrano-Bueno et al. 2020. Detection has to be done on a nuclear extract preparation in the evening on a Long Day that is the moment when CO protein is more stable.
background:	CO (CONSTANS) is a putative transcription factor which is involved in the long day flowering pathway and has been implied to control mediation between circadian clock and flowering. It regulates flowering time by interactions with SOC1 (suppressor of CO 1), TF1 (terminal flower 1), and FT (flowering locus T). Furthermore it is involved in proline and ethylene biosynthesis.
calculated \| apparent molecular mass [kDa]:	41,9 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Chicken
immunogen:	KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CO protein, UniProt: Q39057, TAIR: At5g15840
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Quantity:	50 µg
recommended dilution:	1: 1000 (WB)
Reconstitution:	for reconstitution please add 50 µl of dest, water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Confirmed reactivity:	Arabidopsis thaliana (recombinant CO)
predicted reactivity:	Camelina sativa, Chrysanthemum sp., Solanum lycopersicum
not reactive in:	No confirmed exceptions from predicted reactivity are currently known