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CO | Constans (chicken antibody)

AS16 3958 | Clonality: Polyclonal | Host: Chicken | Reactivity: Arabidopsis thaliana

CO | Constans (chicken antibody) in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS16 3958)
CO | Constans (chicken antibody)



DATA SHEET IN PDF

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Data sheet Product citations Protocols Add review
 

Product Information

Immunogen KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CO protein, UniProt: Q39057, TAIR: At5g15840
Host Chicken
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution for reconstitution please add 50 ĩl of dest, water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1: 1000 (WB)
Expected | apparent MW 41,9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana (recombinant CO)
Predicted reactivity Camelina sativa, Chrysanthemum sp., Solanum lycopersicum
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Recommended protocol for CO protein extraction Serrano-Bueno et al. 2020. Detection has to be done on a nuclear extract preparation in the evening on a Long Day that is the moment when CO protein is more stable.

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AS12 1864A | anti-GI | Gigantea, rabbit polyclonal antibody
AS12 2646 | anti-TOC1 | Timing of CAB expression 1, rabbit polyclonal antibody
AS14 2823 | anti-TOC1 | Timing of CAB expression 1 (other species), rabbit polyclonal antibody
AS06 198 | anti-FT/TSF | Flowering locus T and twin sister of FT, rabbit polyclonal antibody
AS12 1856 | anti-SOC1 | Suppressor of constans overexpression 1, rabbit polyclonal antibody

Plant protein extraction buffer

Secondary antibodies

Background

Background CO (CONSTANS) is a putative transcription factor which is involved in the long day flowering pathway and has been implied to control mediation between circadian clock and flowering. It regulates flowering time by interactions with SOC1 (suppressor of CO 1), TF1 (terminal flower 1), and FT (flowering locus T). Furthermore it is involved in proline and ethylene biosynthesis.

Product citations

Selected references To be added when available, antibody released in February 2021.

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