CO | Constans (goat antibody)

Product no: AS16 3160

AS16 3160 | Clonality: Polyclonal | Host: Goat | Reactivity: Arabidopsis thaliana

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  • Data sheet
  •  
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CO protein, UniProt: Q39057, TAIR: At5g15840
    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: for reconstitution please add 50 l of dest, water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1: 1000 (WB)
    Expected | apparent MW: 41,9 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana (recombianant CO)
    Predicted reactivity: Camelina sativa, Chrysanthemum sp.
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: Antibody is recognizing recombinant CO protein. Detection conditions in endogenous extract remain to be determined.

    Recommended protocol for CO protein extraction Serrano-Bueno et al. 2020. Detection has to be done on a nuclear extract preparation in the evening on a Long Day that is the moment when CO protein is more stable.
  • Background
  • Background: CO (CONSTANS) is a putative transcription factor which is involved in the long day flowering pathway and has been implied to control mediation between circadian clock and flowering. It regulates flowering time by interactions with SOC1 (suppressor of CO 1), TF1 (terminal flower 1), and FT (flowering locus T). Furthermore it is involved in proline and ethylene biosynthesis.
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