Anti-CO | Constans (goat antibody)

Name: Anti-CO | Constans (goat antibody)
SKU: AS16 3160
Price: 235 EUR
Availability: InStock

Product no: AS16 3160

AS16 3160 | Clonality: Polyclonal | Host: Goat | Reactivity: Arabidopsis thaliana

235 €

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DATA SHEET IN PDF

Delivery: 3-6 business days

Data sheet
Product Info

Immunogen:	KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CO protein, UniProt: Q39057, TAIR: At5g15840
Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
Reconstitution:	For reconstitution please add 50 µl of dest, water
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1: 1000 (WB)
Expected \| apparent MW:	41.9 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana (recombianant CO)
Predicted reactivity:	Camelina sativa, Chrysanthemum sp. Species of your interest not listed? Contact us
Not reactive in:	Brassica oleracea var. oleracea, Brassica rapa, Raphanus sativus, Vitis vinifera

Additional Information

Additional information:

Antibody is recognizing recombinant CO protein. Detection conditions in endogenous extract remain to be determined.

Recommended protocol for CO protein extraction Serrano-Bueno et al. 2020. Detection has to be done on a nuclear extract preparation in the evening on a Long Day that is the moment when CO protein is more stable.

Background

Background:

CO (CONSTANS) is a putative transcription factor which is involved in the long day flowering pathway and has been implied to control mediation between circadian clock and flowering. It regulates flowering time by interactions with SOC1 (suppressor of CO 1), TF1 (terminal flower 1), and FT (flowering locus T). Furthermore it is involved in proline and ethylene biosynthesis.

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